Rabbit Polyclonal Phospho-Lfc (S885) antibody (STJ90558)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Lfc (S885) antibody (STJ90558)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Lfc (phospho Ser885) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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ARHGEF2 antibody, KIAA0651 antibody, LFP40 antibody,|AA408978 antibody|ARHG2 antibody|ARHG2_HUMAN antibody|ARHGEF 2 antibody|ARHGEF-2 antibody|ARHGEF2 antibody|GEF antibody|GEF H1 antibody|GEF-H1 antibody|GEFH1 antibody|Guanine nucleotide exchange factor H1 antibody|KIAA0651 antibody|Lbcl1 antibody|Lfc antibody|LFP40 antibody|MGC95068 antibody|Microtubule-regulated Rho-GEF antibody|mKIAA0651 antibody|P40 antibody|Proliferating cell nucleolar antigen p40 antibody|Protein GEF-H1 antibody|Rho guanine nucleotide exchange factor 2 antibody|rho/rac guanine nucleotide exchange factor (GEF) 2 antibody|rho/rac guanine nucleotide exchange factor 2 antibody|rho/rac guanine nucleotide exchange factor antibody|Anti-GEF H1 antibody (ab155785)
SCBT cat No: sc-134827|sc-49684|

 

Name

Lfc (phospho Ser885) Polyclonal Antibody

Catalogue No.

STJ90558

Reactivity

Human, Mouse, Rat

Specificity

Phospho-Lfc (S885) Polyclonal Antibody detects endogenous levels of Lfc protein only when phosphorylated at S885.

Immunogen

Synthesized phospho-peptide derived from Lfc (phospho Ser885) at AA range 820-900

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

111 kDa

Formulation

Lfc (phospho Ser885) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Lfc (phospho Ser885) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Rho guanine nucleotide exchange factor 2 antibody, Guanine nucleotide exchange factor H1 antibody, GEF-H1 antibody, Microtubule-regulated Rho-GEF antibody, Proliferating cell nucleolar antigen p40 antibody

Database links

Human UniProt/Swiss-Prot:Q92974;Mouse UniPort/Swiss-Prot: Q60875;Rat UniProt/Swiss-Port: Q5FVC2;Human Entrez Gene: 9181;Mouse Entrez Gene: 16800;Rat Entrez Gene: Rn.12255

Protein names

Rho guanine nucleotide exchange factor 2 , Guanine nucleotide exchange factor H1 , GEF-H1 , Microtubule-regulated Rho-GEF , Proliferating cell nucleolar antigen p40

Protein function

Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2.

Protein tissue specificity

Up-regulated by bacterial peptidoglycans stimulation, such as muramyl dipeptide and in biopsies from inflamed mucosal areas of Crohn’s disease patients.

Protein sequence and domain

The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA. Promotes tyrosine phosphorylation of RIPK2. / The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions. / Contains 1 DH (DBL-homology) domain. / Contains 1 PH domain. / Contains 1 phorbol-ester/DAG-type zinc finger.

Protein post-translational modifications

Phosphorylation of Ser-886 by PAK1 induces binding to protein YWHAZ, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by AURKA and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules. Phosphorylated on serine, threonine and tyrosine residues in a RIPK2-dependent manner.

Protein cellular localization

Cytoplasm / Cell junction > tight junction / Golgi apparatus / Cytoplasm > cytoskeleton > spindle / Cell projection > ruffle membrane / Cytoplasmic vesicle

Research area

All research areas>GDP/GTP Binding Proteins>Lfc
(View all antibody categories related to GDP/GTP Binding Proteins)

Note

AntibodyPlus can customize Lfc (phospho Ser885) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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