Rabbit Polyclonal Phospho-LIMK-1/2 (T508/505) antibody (STJ90751)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-LIMK-1/2 (T508/505) antibody (STJ90751)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for LIMK-1/2 (phospho Thr508/505) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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LIMK1 antibody, LIMK antibody,|EC 2.7.1.37 antibody|LIM domain containing protein kinase antibody|LIM domain kinase 1 antibody|LIM kinase antibody|LIM motif containing protein kinase antibody|LIMK 1 antibody|LIMK antibody|LIMK-1 antibody|limk1 antibody|LIMK1_HUMAN antibody|Phospho anti-LIM Kinase 1 (T508) antibody (ab38508)
SCBT cat No: sc-135973|sc-8387|sc-48346|sc-515585|sc-5576|

 

Name

LIMK-1/2 (phospho Thr508/505) Polyclonal Antibody

Catalogue No.

STJ90751

Reactivity

Human, Mouse, Rat

Specificity

Phospho-LIMK-1/2 (T508/505) Polyclonal Antibody detects endogenous levels of LIMK-1/2 protein only when phosphorylated at T508/505.

Immunogen

Synthesized phospho-peptide derived from LIMK-1/2 (phospho Thr508/505) at AA range 450-530

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

72 kDa

Formulation

LIMK-1/2 (phospho Thr508/505) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

LIMK-1/2 (phospho Thr508/505) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

LIM domain kinase 1 antibody, LIMK-1 antibody,

Database links

Human UniProt/Swiss-Prot:P53667 ;Human Entrez Gene: 3984;

Protein names

LIM domain kinase 1 , LIMK-1 ,

Protein function

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1). / ATP + a protein = ADP + a phosphoprotein.

Protein tissue specificity

Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

Involvement in disease

Note: LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

Protein sequence and domain

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. / Contains 2 LIM zinc-binding domains. / Contains 1 PDZ (DHR) domain. / Contains 1 protein kinase domain.

Protein post-translational modifications

Autophosphorylated (By similarity). Phosphorylated on Thr-508 by ROCK1 and PAK1, resulting in activation. Phosphorylated by PAK4 which increases the ability of LIMK1 to phosphorylate cofilin. Phosphorylated at Ser-323 by MAPKAPK2 during activation of VEGFA-induced signaling, which results in activation of LIMK1 and promotion of actin reorganization, cell migration, and tubule formation of endothelial cells. Dephosphorylated and inactivated by SSH1. Phosphorylated by CDC42BP. / Ubiquitinated. ‘Lys-48’-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM (By similarity).

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Kinases and Phosphatases>LIMK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize LIMK-1/2 (phospho Thr508/505) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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