Rabbit Polyclonal Phospho-MAP2K1-S217/MAP2K2-S221 antibody (AP0209)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-MAP2K1-S217/MAP2K2-S221 antibody (AP0209)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:1000
IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-MAP2K1-S217/MAP2K2-S221 antibody.

Alternative names:

Check alternative names for the antibody

Expand

CFC3/CFC4 antibody; MEK1/MEK2 antibody; MKK1/MKK2 antibody; MAPKK1/MAPKK2 antibody; PRKMK1/PRKMK2 antibody;
Dual specificity mitogen activated protein kinase kinase 1 antibody|Dual specificity mitogen-activated protein kinase kinase 1 antibody|ERK activator kinase 1 antibody|MAP kinase kinase 1 antibody|MAP kinase/Erk kinase 1 antibody|MAP2K1 antibody|MAPK/ERK kinase 1 antibody|MAPKK 1 antibody|MAPKK1 antibody|MEK 1 antibody|Mek1 antibody|MEKK1 antibody|Mitogen activated protein kinase kinase 1 antibody|MKK 1 antibody|MKK1 antibody|MP2K1_HUMAN antibody|PRKMK1 antibody|Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody|Protein kinase mitogen activated, kinase 1 antibody|Phospho anti-MEK1 (S298) antibody [EPR3338] (ab96379)

SCBT cat No: sc-365800|sc-219|sc-6250|sc-436|sc-81504|sc-81473|sc-136261|sc-13159|sc-525|sc-524|sc-135985|

Name

Rabbit Polyclonal Phospho-MAP2K1-S217/MAP2K2-S221 antibody

Catalogue No.

AP0209

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding serine 217/221 of human MAP2K1/MAP2K2.

Host

Rabbit

Applications

WB, IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

45kDa

Formulation

Phospho-MAP2K1-S217/MAP2K2-S221 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CFC3/CFC4 antibody; MEK1/MEK2 antibody; MKK1/MKK2 antibody; MAPKK1/MAPKK2 antibody; PRKMK1/PRKMK2 antibody;

Protein names

CFC3/CFC4; MEK1/MEK2; MKK1/MKK2; MAPKK1/MAPKK2; PRKMK1/PRKMK2;

Protein function

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Protein tissue specificity

Widely expressed, with extremely low levels in brain.

Protein sequence and domain

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.; Contains 1 protein kinase domain. The proline-rich region localized between residues 270 and 307 is important for binding to RAF1 and activation of MAP2K1/MEK1.

Protein post-translational modifications

Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK.; Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.

Protein cellular localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body . Cytoplasm . Nucleus . Membrane ; Peripheral membrane protein . Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111). Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742).

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Kinases-and-Phosphatases/">Kinases and Phosphatases</a>><a href="http://antibodyplus.com/product-tag/MEK/">MEK</a><br><a href="http://antibodyplus.com/product-tag/Kinases-and-Phosphatases/"&gt; (View all antibody categories related to Kinases and Phosphatases)</a>

Note

AntibodyPlus can customize Phospho-MAP2K1-S217/MAP2K2-S221 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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