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Rabbit Polyclonal Phospho-MAP3K5-S966 antibody (AP0194)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC
WB 1:500 – 1:2000
IHC 1:50 – 1:100
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit Polyclonal Phospho-MAP3K5-S966 antibody.
Check alternative names for the antibodyExpand
ASK1 antibody; MEKK5 antibody; MAPKKK5 antibody;
Apoptosis signal regulating kinase 1 antibody|Apoptosis signal-regulating kinase 1 antibody|ASK 1 antibody|ASK-1 antibody|ASK1 antibody|M3K5 antibody|M3K5_HUMAN antibody|MAP/ERK kinase kinase 5 antibody|MAP3K5 antibody|MAPK/ERK kinase kinase 5 antibody|MAPKKK5 antibody|MEK kinase 5 antibody|MEKK 5 antibody|MEKK5 antibody|Mitogen activated protein kinase kinase kinase 5 antibody|Mitogen-activated protein kinase kinase kinase 5 antibody|Anti-ASK1 antibody [EP553Y] (ab45178)
SCBT cat No: sc-390397|sc-5294|sc-390275|sc-7931|sc-49371|sc-6368|sc-166787|sc-374353|sc-68250|sc-293189|
Rabbit Polyclonal Phospho-MAP3K5-S966 antibody
|Catalogue No.|| |
A phospho specific peptide corresponding to residues surrounding S966 of human MAP3K5
|Recommended dilution|| |
WB 1:500 – 1:2000<br/>IHC 1:50 – 1:100
|Molecular weight|| |
Phospho-MAP3K5-S966 antibody was tube-contained.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
ASK1 antibody; MEKK5 antibody; MAPKKK5 antibody;
|Database links|| |
Human UniProt/Swiss-Prot:<a href="http://www.uniprot.org/uniprot/Q99683">Q99683</a>
|Protein names|| |
ASK1; MEKK5; MAPKKK5;
|Protein function|| |
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
|Protein tissue specificity|| |
Abundantly expressed in heart and pancreas.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.; Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylated at Thr-838 through autophosphorylation and by MAP3K6/ASK2 which leads to activation. Thr-838 is dephosphorylated by PPP5C. Ser-83 and Ser-1033 are inactivating phosphorylation sites, the former of which is phosphorylated by AKT1 and AKT2. Phosphorylated at Ser-966 which induces association of MAP3K5/ASK1 with the 14-3-3 family proteins and suppresses MAP3K5/ASK1 activity. Calcineurin (CN) dephosphorylates this site. Also dephosphorylated and activated by PGAM5.; Ubiquitinated. Tumor necrosis factor (TNF) induces TNFR2-dependent ubiquitination leading to proteasomal degradation.
|Protein cellular localization|| |
Cytoplasm. Endoplasmic reticulum. Note=Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Kinases-and-Phosphatases/">Kinases and Phosphatases</a>><a href="http://antibodyplus.com/product-tag/ASK-1/">ASK 1</a><br><a href="http://antibodyplus.com/product-tag/Kinases-and-Phosphatases/"> (View all antibody categories related to Kinases and Phosphatases)</a>
AntibodyPlus can customize Phospho-MAP3K5-S966 antibody according to your requirement, including bulk product size,etc. Please contact firstname.lastname@example.org. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
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