Rabbit polyclonal Phospho-MAP3K7-S412 antibody (AP0071)

$69.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-MAP3K7-S412 antibody (AP0071)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-MAP3K7-S412 antibody.

Alternative names:

Check alternative names for the antibody

Expand

TAK1 antibody, MEKK7 antibody, TGF1a antibody
M3K7_HUMAN antibody|MAP3K 7 antibody|Map3k7 antibody|MEKK7 antibody|Mitogen activated protein kinase kinase kinase 7 antibody|Mitogen-activated protein kinase kinase kinase 7 antibody|TAK1 antibody|TGF beta activated kinase 1 antibody|TGF-beta-activated kinase 1 antibody|TGF1a antibody|Transforming growth factor beta activated kinase 1 antibody|Transforming growth factor-beta-activated kinase 1 antibody|Anti-TAK1 antibody [EPR5984] (ab109526)

SCBT cat No: sc-130219|sc-248320|sc-71794|sc-32275|sc-32276|

Name

Rabbit polyclonal Phospho-MAP3K7-S412 antibody

Catalogue No.

AP0071

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S412 of human MAP3K7

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 67kDa/Observed: Refer to Figures

Formulation

Phospho-MAP3K7-S412 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-MAP3K7-S412 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

TAK1 antibody, MEKK7 antibody, TGF1a antibody

Database links

Human UniProt/Swiss-Prot:O43318

Protein names

TAK1, MEKK7, TGF1a

Protein function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity.

Protein tissue specificity

Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. Isoform 1B is highly expressed in brain, kidney and small intestine. Isoform 1C is the major form in prostate. Isoform 1D is the less abundant form.

Protein sequence and domain

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.; Contains 1 protein kinase domain.

Protein post-translational modifications

Association with TAB1/MAP3K7IP1 promotes autophosphorylation at Ser-192 and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Ser-192 by PPM1B/PP2CB and at Thr-187 by PP2A and PPP6C leads to inactivation.; ‘Lys-48’-linked polyubiquitination at Lys-72 is induced by TNFalpha, and leads to proteasomal degradation. Requires ‘Lys-63’-linked polyubiquitination for autophosphorylation and subsequent activation. ‘Lys-63’-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves ‘Lys-63’-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.

Protein cellular localization

Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Note: Although the majority of MAP3K7/TAK1 is found in the cytosol, when complexed with TAB1/MAP3K7IP1 and TAB2/MAP3K7IP2, it is also localized at the cell membrane.

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Research area

All research areas>Kinases and Phosphatases>Tak
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Phospho-MAP3K7-S412 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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