Rabbit polyclonal Phospho-MAPK14-Y322 antibody (AP0055)

$69.00$259.00

Reactivity: Human
Applications: WB,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-MAPK14-Y322 antibody (AP0055)

Supplier: ABclonal Inc.

Recommended applications: WB,IF

Recommended dilution:

WB 1:500 – 1:2000 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-MAPK14-Y322 antibody.

Alternative names:

Check alternative names for the antibody

Expand

RK antibody, p38 antibody, CSBP antibody, EXIP antibody, Mxi2 antibody, CSBP1 antibody, CSBP2 antibody, CSPB1 antibody, PRKM14 antibody, PRKM15 SAPK2A antibody, p38ALPHA antibody
CSAID Binding Protein 1 antibody|CSAID binding protein antibody|CSAID-binding protein antibody|Csaids binding protein antibody|CSBP 1 antibody|CSBP 2 antibody|CSBP antibody|CSBP1 antibody|CSBP2 antibody|CSPB1 antibody|Cytokine suppressive anti-inflammatory drug-binding protein antibody|EXIP antibody|MAP kinase 14 antibody|MAP kinase MXI2 antibody|MAP kinase p38 alpha antibody|MAPK 14 antibody|MAPK14 antibody|MAX interacting protein 2 antibody|MAX-interacting protein 2 antibody|Mitogen Activated Protein Kinase 14 antibody|Mitogen activated protein kinase p38 alpha antibody|Mitogen-activated protein kinase 14 antibody|Mitogen-activated protein kinase p38 alpha antibody|MK14_HUMAN antibody|Mxi 2 antibody|MXI2 antibody|p38 ALPHA antibody|p38 antibody|p38 MAP kinase antibody|p38 MAPK antibody|p38 mitogen activated protein kinase antibody|p38ALPHA antibody|p38alpha Exip antibody|PRKM14 antibody|PRKM15 antibody|RK antibody|SAPK2A antibody|Anti-p38 antibody [E229] (ab170099)

SCBT cat No: sc-136210|sc-81621|sc-33688|sc-535|sc-398305|sc-271120|sc-166357|sc-728|sc-7972|sc-7149|sc-101427|sc-33689|sc-6176|sc-6187|

Name

Rabbit polyclonal Phospho-MAPK14-Y322 antibody

Catalogue No.

AP0055

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding Y322 of human MAPK14

Host

Rabbit

Applications

WB, IF

Recommended dilution

WB 1:500 – 1:2000
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: /Observed: Refer to Figures

Formulation

Phospho-MAPK14-Y322 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-MAPK14-Y322 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

RK antibody, p38 antibody, CSBP antibody, EXIP antibody, Mxi2 antibody, CSBP1 antibody, CSBP2 antibody, CSPB1 antibody, PRKM14 antibody, PRKM15 SAPK2A antibody, p38ALPHA antibody

Database links

Human UniProt/Swiss-Prot:Q16539

Protein names

RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15 SAPK2A, p38ALPHA

Protein function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on ‘Ser-10’ (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at ‘Thr-113’.

Protein tissue specificity

Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.

Protein sequence and domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.; Contains 1 protein kinase domain.

Protein post-translational modifications

Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory citokines, environmental stress or growth factors, which activates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16. PPM1D also mediates dephosphorylation and inactivation of MAPK14 .; Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3.; Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.

Protein cellular localization

Cytoplasm . Nucleus .

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38alpha, beta, gamma (also known as Erk6 or SAPK3), and delta (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

Research area

All research areas>Kinases and Phosphatases>p38
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Phospho-MAPK14-Y322 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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