Rabbit Polyclonal Phospho-MAPKAPK-2 (T222) antibody (STJ90852)

$99.00$319.00

Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-MAPKAPK-2 (T222) antibody (STJ90852)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for MAPKAPK-2 (phospho Thr222) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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MAPKAPK2 antibody,|MAP kinase activated protein Kinase 2 antibody|MAP kinase-activated protein kinase 2 antibody|MAPK activated protein kinase 2 antibody|MAPK-activated protein kinase 2 antibody|MAPK2_HUMAN antibody|MAPKAP K2 antibody|MAPKAP kinase 2 antibody|MAPKAPK 2 antibody|MAPKAPK-2 antibody|MAPKAPK2 antibody|Mitogen activated protein kinase activated protein kinase 2 antibody|MK 2 antibody|MK2 antibody|Phospho anti-MAPKAP Kinase 2 (T334) antibody (ab63378)
SCBT cat No: sc-100393|sc-393609|sc-6221|sc-7871|sc-514754|sc-164966|sc-104369|sc-100510|

 

Name

MAPKAPK-2 (phospho Thr222) Polyclonal Antibody

Catalogue No.

STJ90852

Reactivity

Human, Mouse, Rat, Monkey

Specificity

Phospho-MAPKAPK-2 (T222) Polyclonal Antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at T222.

Immunogen

Synthesized phospho-peptide derived from MAPKAPK-2 (phospho Thr222) at AA range 160-240

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

45 kDa

Formulation

MAPKAPK-2 (phospho Thr222) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

MAPKAPK-2 (phospho Thr222) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

MAP kinase-activated protein kinase 2 antibody, MAPK-activated protein kinase 2 antibody, MAPKAP kinase 2 antibody, MAPKAP-K2 antibody, MAPKAPK-2 antibody, MK-2 antibody, MK2 antibody,

Database links

Human UniProt/Swiss-Prot:P49137;Mouse UniPort/Swiss-Prot: P49138;Rat UniProt/Swiss-Port: Q80ZF4;Human Entrez Gene: 9261;Mouse Entrez Gene: 17164;Rat Entrez Gene: Rn.6276

Protein names

MAP kinase-activated protein kinase 2 , MAPK-activated protein kinase 2 , MAPKAP kinase 2 , MAPKAP-K2 , MAPKAPK-2 , MK-2 , MK2 ,

Protein function

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. / ATP + a protein = ADP + a phosphoprotein. / Activated following phosphorylation by p38-alpha/MAPK14 following various stresses. Inhibited following sumoylation. Specifically inhibited by pyrrolopyridine inhibitors.

Protein tissue specificity

Expressed in all tissues examined.

Protein sequence and domain

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. / Contains 1 protein kinase domain.

Protein post-translational modifications

Sumoylation inhibits the protein kinase activity. / Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-222, Ser-272 and Thr-334.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Kinases and Phosphatases>MAPKAPK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize MAPKAPK-2 (phospho Thr222) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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