Rabbit Polyclonal Phospho-MDM2-S166 antibody (AP0073)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-MDM2-S166 antibody (AP0073)

Supplier: ABclonal Inc.

Recommended applications: WB,IF

Recommended dilution:

WB 1:500 – 1:1000
IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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HDMX antibody; hdm2 antibody; ACTFS antibody;
ACTFS antibody|Double minute 2 protein antibody|E3 ubiquitin-protein ligase Mdm2 antibody|Hdm 2 antibody|Hdm2 antibody|HDMX antibody|MDM 2 antibody|MDM2 antibody|MDM2 oncogene E3 ubiquitin protein ligase antibody|Mdm2 p53 E3 ubiquitin protein ligase homolog antibody|Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody|MDM2_HUMAN antibody|MDM2BP antibody|Mouse Double Minute 2 antibody|MTBP antibody|Murine Double Minute Chromosome 2 antibody|Oncoprotein Mdm2 antibody|p53 Binding Protein Mdm2 antibody|p53-binding protein Mdm2 antibody|Ubiquitin protein ligase E3 Mdm2 antibody|Ubiquitin protein ligase E3 Mdm2 antibody|Anti-MDM2 antibody [2A10] (ab16895)

SCBT cat No: sc-812|sc-5304|sc-13161|sc-7918|sc-56154|sc-813|sc-965|sc-56430|

Name

Rabbit Polyclonal Phospho-MDM2-S166 antibody

Catalogue No.

AP0073

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding S166 of human MDM2

Host

Rabbit

Applications

WB, IF

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

55kDa

Formulation

Phospho-MDM2-S166 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

HDMX antibody; hdm2 antibody; ACTFS antibody;

Protein names

HDMX; hdm2; ACTFS;

Protein function

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity).

Protein tissue specificity

Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.

Protein sequence and domain

Belongs to the MDM2/MDM4 family.; Contains 1 RanBP2-type zinc finger.; Contains 1 RING-type zinc finger.; Contains 1 SWIB domain. Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.

Protein post-translational modifications

Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53. Phosphorylated at multiple sites near the RING domain by ATM upon DNA damage; this prevents oligomerization and E3 ligase processivity and impedes constitutive p53/TP53 degradation.; Autoubiquitination leads to proteasomal degradation; resulting in p53/TP53 activation it may be regulated by SFN. Also ubiquitinated by TRIM13. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitination and degradation of p53/TP53. Deubiquitinated by USP7 leading to its stabilization.

Protein cellular localization

Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.

Background

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Tumor-SuppressorApoptosis/">Tumor Suppressor/Apoptosis</a>><a href="http://antibodyplus.com/product-tag/MDM2/">MDM2</a><br><a href="http://antibodyplus.com/product-tag/Tumor-SuppressorApoptosis/"&gt; (View all antibody categories related to Tumor Suppressor/Apoptosis)</a>

Note

AntibodyPlus can customize Phospho-MDM2-S166 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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