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Rabbit Polyclonal Phospho-MDM2 (S166) antibody (STJ91043)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
MDM2 antibody,|ACTFS antibody|Double minute 2 protein antibody|E3 ubiquitin-protein ligase Mdm2 antibody|Hdm 2 antibody|Hdm2 antibody|HDMX antibody|MDM 2 antibody|MDM2 antibody|MDM2 oncogene E3 ubiquitin protein ligase antibody|Mdm2 p53 E3 ubiquitin protein ligase homolog antibody|Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody|MDM2_HUMAN antibody|MDM2BP antibody|Mouse Double Minute 2 antibody|MTBP antibody|Murine Double Minute Chromosome 2 antibody|Oncoprotein Mdm2 antibody|p53 Binding Protein Mdm2 antibody|p53-binding protein Mdm2 antibody|Ubiquitin protein ligase E3 Mdm2 antibody|Ubiquitin protein ligase E3 Mdm2 antibody|Anti-MDM2 antibody [2A10] (ab16895)
SCBT cat No: sc-812|sc-5304|sc-13161|sc-7918|sc-56154|sc-813|sc-965|sc-56430|
MDM2 (phospho Ser166) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Monkey
Phospho-MDM2 (S166) Polyclonal Antibody detects endogenous levels of MDM2 protein only when phosphorylated at S166.
Synthesized phospho-peptide derived from MDM2 (phospho Ser166) at AA range 110-190
WB, IHC, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;
|Molecular weight|| |
MDM2 (phospho Ser166) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
MDM2 (phospho Ser166) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
E3 ubiquitin-protein ligase Mdm2 antibody, Double minute 2 protein antibody, Hdm2 antibody, Oncoprotein Mdm2 antibody, p53-binding protein Mdm2 antibody
|Protein names|| |
E3 ubiquitin-protein ligase Mdm2 , Double minute 2 protein , Hdm2 , Oncoprotein Mdm2 , p53-binding protein Mdm2
|Protein function|| |
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation.
|Protein tissue specificity|| |
Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
|Involvement in disease|| |
Note: Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
|Protein sequence and domain|| |
Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself. / Belongs to the MDM2/MDM4 family. / Contains 1 RanBP2-type zinc finger. / Contains 1 RING-type zinc finger. / Contains 1 SWIB domain.
|Protein post-translational modifications|| |
Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53. Phosphorylated at multiple sites near the RING domain by ATM upon DNA damage; this prevents oligomerization and E3 ligase processivity and impedes constitutive p53/TP53 degradation. / Autoubiquitination leads to proteasomal degradation; resulting in p53/TP53 activation it may be regulated by SFN. Also ubiquitinated by TRIM13. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitination and degradation of p53/TP53. Deubiquitinated by USP7 leading to its stabilization.
|Protein cellular localization|| |
Nucleus > nucleoplasm / Cytoplasm / Nucleus > nucleolus
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St John’s Laboratory Ltd.
|Product type|| |
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