Rabbit Polyclonal Phospho-MEF2A-T319 antibody (AP0407)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-MEF2A-T319 antibody (AP0407)

Supplier: ABclonal Inc.

Recommended applications: IF

Recommended dilution:

IF 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

mef2 antibody; ADCAD1 antibody; RSRFC4 antibody; RSRFC9 antibody;
ADCAD1 antibody|MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody|MEF2 antibody|MEF2A antibody|MEF2A_HUMAN antibody|Myocyte enhancer factor 2A antibody|Myocyte-specific enhancer factor 2A antibody|RSRFC4 antibody|RSRFC9 antibody|Serum response factor like protein 1 antibody|Serum response factor-like protein 1 antibody|Phospho anti-MEF2A (T312) antibody (ab30644)

SCBT cat No: sc-313|sc-13917|sc-293456|sc-293457|sc-17785|sc-55500|sc-10794|sc-376504|

Name

Rabbit Polyclonal Phospho-MEF2A-T319 antibody

Catalogue No.

AP0407

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding T319 of human MEF2A

Host

Rabbit

Applications

IF

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

54kDa

Formulation

Phospho-MEF2A-T319 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

mef2 antibody; ADCAD1 antibody; RSRFC4 antibody; RSRFC9 antibody;

Protein names

mef2; ADCAD1; RSRFC4; RSRFC9;

Protein function

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.

Protein tissue specificity

Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.

Protein sequence and domain

Belongs to the MEF2 family.; Contains 1 MADS-box domain.; Contains 1 Mef2-type DNA-binding domain.

Protein post-translational modifications

Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.; Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated with SUMO1 and, to a lesser extent with SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis (By similarity).; Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.; Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.

Protein cellular localization

Nucleus .

Background

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/MEF/">MEF</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"&gt; (View all antibody categories related to Transcription Regulators)</a>

Note

AntibodyPlus can customize Phospho-MEF2A-T319 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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