Rabbit Polyclonal Phospho-MEK-1/2 (S218/222) antibody (STJ90327)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-MEK-1/2 (S218/222) antibody (STJ90327)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for MEK-1/2 (phospho Ser218/222) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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MAP2K1 antibody, MEK1 antibody, PRKMK1 antibody,|Dual specificity mitogen activated protein kinase kinase 1 antibody|Dual specificity mitogen-activated protein kinase kinase 1 antibody|ERK activator kinase 1 antibody|MAP kinase kinase 1 antibody|MAP kinase/Erk kinase 1 antibody|MAP2K1 antibody|MAPK/ERK kinase 1 antibody|MAPKK 1 antibody|MAPKK1 antibody|MEK 1 antibody|Mek1 antibody|MEKK1 antibody|Mitogen activated protein kinase kinase 1 antibody|MKK 1 antibody|MKK1 antibody|MP2K1_HUMAN antibody|PRKMK1 antibody|Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody|Protein kinase mitogen activated, kinase 1 antibody|Phospho anti-MEK1 (S298) antibody [EPR3338] (ab96379)
SCBT cat No: sc-365800|sc-219|sc-6250|sc-436|sc-81504|sc-81473|sc-136261|sc-13159|sc-525|sc-524|sc-135985|

Name

MEK-1/2 (phospho Ser218/222) Polyclonal Antibody

Catalogue No.

STJ90327

Reactivity

Human, Mouse, Rat

Specificity

Phospho-MEK-1/2 (S218/222) Polyclonal Antibody detects endogenous levels of MEK-1/2 protein only when phosphorylated at S218/222.

Immunogen

Synthesized phospho-peptide derived from MEK-1/2 (phospho Ser218/222) at AA range 160-240

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

48 kDa

Formulation

MEK-1/2 (phospho Ser218/222) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

MEK-1/2 (phospho Ser218/222) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Dual specificity mitogen-activated protein kinase kinase 1 antibody, MAP kinase kinase 1 antibody, MAPKK 1 antibody, MKK1 antibody, ERK activator kinase 1 antibody, MAPK/ERK kinase 1 antibody, MEK 1 antibody

Database links

Human UniProt/Swiss-Prot:Q02750 ;Human Entrez Gene: 5604;

Protein names

Dual specificity mitogen-activated protein kinase kinase 1 , MAP kinase kinase 1 , MAPKK 1 , MKK1 , ERK activator kinase 1 , MAPK/ERK kinase 1 , MEK 1

Protein function

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. / ATP + a protein = ADP + a phosphoprotein. / Ras proteins such as HRAS mediate the activation of RAF proteins such as RAF1 or BRAF which in turn activate extracellular signal-regulated kinases (ERK) through MAPK (mitogen-activated protein kinases) and ERK kinases MAP2K1/MEK1 and MAP2K2/MEK2. Activation occurs through phosphorylation of Ser-218 and Ser-222. MAP2K1/MEK1 is also the target of negative feed-back regulation by its substrate kinases, such as MAPK1/ERK2. These phosphorylate MAP2K1/MEK1 on Thr-292, thereby facilitating dephosphorylation of the activating residues Ser-218 and Ser-222. Inhibited by serine/threonine phosphatase 2A (By similarity). Many inhibitors have been identified including pyrrole derivatives, TAK-733 (one of a series of 8-methylpyrido[2,3-d]pyrimidine-4,7(3H,8H)-dione derivatives), CH4987655 and RDEA119/BAY 869766.

Protein tissue specificity

Widely expressed, with extremely low levels in brain.

Involvement in disease

Cardiofaciocutaneous syndrome 3 (CFC3) [MIM:615279]: A form of cardiofaciocutaneous syndrome, a multiple congenital anomaly disorder characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. Distinctive features of CFC3 include macrostomia and horizontal shape of palpebral fissures. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The proline-rich region localized between residues 270 and 307 is important for binding to RAF1 and activation of MAP2K1/MEK1. / Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. / Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.

Protein cellular localization

Cytoplasm > cytoskeleton > microtubule organizing center > centrosome / Cytoplasm > cytoskeleton > microtubule organizing center > spindle pole body / Cytoplasm / Nucleus / Membrane / Peripheral membrane protein

Research area

All research areas>Kinases and Phosphatases>MEK
(View all antibody categories related to Kinases and Phosphatases)

Note

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Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunohistochemical analysis of paraffin embedded Human liver tissue

1: MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat brain tissue

1: MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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