Rabbit Polyclonal Phospho-MEK-4 (T261) antibody (STJ90332)

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Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-MEK-4 (T261) antibody (STJ90332)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for MEK-4 (phospho Thr261) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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MAP2K4 antibody, JNKK1 antibody, MEK4 antibody, MKK4 antibody, PRKMK4 antibody, SEK1 antibody, SERK1 antibody, SKK1 antibody,|c Jun N terminal kinase kinase 1 antibody|C-JUN N-terminal kinase kinase 1 antibody|Dual specificity mitogen activated protein kinase kinase 4 antibody|Dual specificity mitogen-activated protein kinase kinase 4 antibody|JNK Activated Kinase 1 antibody|JNK activating kinase 1 antibody|JNK-activating kinase 1 antibody|JNKK antibody|JNKK1 antibody|MAP kinase kinase 4 antibody|Map2k4 antibody|MAPK ERK kinase 4 antibody|MAPK/ERK kinase 4 antibody|MAPKK 4 antibody|MAPKK4 antibody|MEK 4 antibody|MEK4 antibody|Mitogen activated protein kinase kinase 4 antibody|MKK 4 antibody|MKK4 antibody|MP2K4_HUMAN antibody|PRKMK4 antibody|SAPK ERK kinase 1 antibody|SAPK/ERK kinase 1 antibody|SAPKK 1 antibody|SAPKK1 antibody|SEK1 antibody|SERK1 antibody|SKK1 antibody|Stress activated protein kinase kinase 1 antibody|Phospho anti-MEK4 (S80) antibody (ab39403)
SCBT cat No: sc-136314|sc-837|sc-166168|sc-376838|sc-13070|sc-964|sc-135986|sc-1287|sc-365198|

 

Name

MEK-4 (phospho Thr261) Polyclonal Antibody

Catalogue No.

STJ90332

Reactivity

Human, Mouse, Rat

Specificity

Phospho-MEK-4 (T261) Polyclonal Antibody detects endogenous levels of MEK-4 protein only when phosphorylated at T261.

Immunogen

Synthesized phospho-peptide derived from MEK-4 (phospho Thr261) at AA range 200-280

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

48 kDa

Formulation

MEK-4 (phospho Thr261) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

MEK-4 (phospho Thr261) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Dual specificity mitogen-activated protein kinase kinase 4 antibody, MAP kinase kinase 4 antibody, MAPKK 4 antibody, JNK-activating kinase 1 antibody, MAPK/ERK kinase 4 antibody, MEK 4 antibody, SAPK/ERK kinase 1 antibody, SEK1 antibody, Stress-activated protein kinase kinase 1 antibody, SAPK kinase 1 antibody, SAPKK-1 antibody, SAPKK1 antibody, c-Jun N-terminal kinase kinase 1 antibody, JNKK antibody

Database links

Human UniProt/Swiss-Prot:P45985;Mouse UniPort/Swiss-Prot: P47809;Rat UniProt/Swiss-Port: Q4KSH6;Human Entrez Gene: 6416;Mouse Entrez Gene: 26398;Rat Entrez Gene: Rn.198875

Protein names

Dual specificity mitogen-activated protein kinase kinase 4 , MAP kinase kinase 4 , MAPKK 4 , JNK-activating kinase 1 , MAPK/ERK kinase 4 , MEK 4 , SAPK/ERK kinase 1 , SEK1 , Stress-activated protein kinase kinase 1 , SAPK kinase 1 , SAPKK-1 , SAPKK1 , c-Jun N-terminal kinase kinase 1 , JNKK

Protein function

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14. / ATP + a protein = ADP + a phosphoprotein. / Activated in response to a variety of cellular stresses, including UV and gamma-irradiation, heat shock, hyperosmolarity, T-cell receptor stimulation, peroxide and inflammatory cytokines. Also activated by developmental cues. MAP2K4/MKK4 is activated by the majority of MKKKs, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K7/TAK1, MAP3K10/MLK2, MAP3K11/MLK3, MAP3K12/DLK and MAP3K13/LZK.

Protein tissue specificity

Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

Protein sequence and domain

The DVD domain (residues 364-387) contains a conserved docking site and is found in the mammalian MAP kinase kinases (MAP2Ks). The DVD sites bind to their specific upstream MAP kinase kinase kinases (MAP3Ks) and are essential for activation. / The D domain (residues 34-52) contains a conserved docking site and is required for the binding to MAPK substrates. / Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Activated by phosphorylation on Ser-257 and Thr-261 by MAP kinase kinase kinases (MAP3Ks).

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Kinases and Phosphatases>MEK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize MEK-4 (phospho Thr261) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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