Rabbit Polyclonal Phospho-MITF (S180) antibody (STJ90470)

$99.00$319.00

Reactivity: Human, Mouse, Monkey
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit Polyclonal Phospho-MITF (S180) antibody (STJ90470)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for MITF (phospho Ser180) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

Expand

MITF antibody, BHLHE32 antibody,|BHLHE32 antibody|bHLHe32 antibody|Class E basic helix-loop-helix protein 32 antibody|CMM8 antibody|Homolog of mouse microphthalmia antibody|Mi antibody|Microphthalmia associated transcription factor antibody|Microphthalmia, mouse, homolog of antibody|Microphthalmia-associated transcription factor antibody|MITF antibody|MITF_HUMAN antibody|mitfa antibody|nacre antibody|WS2 antibody|WS2A antibody|z3A.1 antibody|Anti-MiTF antibody [C5] – ChIP Grade (ab12039)
SCBT cat No: sc-52938|sc-71588|sc-71587|sc-11002|sc-56725|sc-56726|sc-25386|sc-10999|sc-56433|

 

Name

MITF (phospho Ser180) Polyclonal Antibody

Catalogue No.

STJ90470

Reactivity

Human, Mouse, Monkey

Specificity

Phospho-MITF (S180) Polyclonal Antibody detects endogenous levels of MITF protein only when phosphorylated at S180.

Immunogen

Synthesized phospho-peptide derived from MITF (phospho Ser180) at AA range 120-200

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

52 kDa

Formulation

MITF (phospho Ser180) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

MITF (phospho Ser180) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Microphthalmia-associated transcription factor antibody, Class E basic helix-loop-helix protein 32 antibody, bHLHe32 antibody

Database links

Human UniProt/Swiss-Prot:O75030;Mouse UniPort/Swiss-Prot: Q08874;Rat UniProt/Swiss-Port: F1LQV3;Human Entrez Gene: 4286;Mouse Entrez Gene: 17342;Rat Entrez Gene: Rn.31427

Protein names

Microphthalmia-associated transcription factor , Class E basic helix-loop-helix protein 32 , bHLHe32

Protein function

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5′-CACGTG-3′) found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Protein tissue specificity

Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.

Involvement in disease

Waardenburg syndrome 2A (WS2A) [MIM:193510]: WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Waardenburg syndrome 2, with ocular albinism, autosomal recessive (WS2-OA) [MIM:103470]: A disorder characterized by the association of features typical of Waardenburg syndrome type 2 with ocular albinism. Patients manifest reduced visual acuity, albinotic fundus, deafness, hypomelanosis. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Tietz syndrome (TIETZS) [MIM:103500]: Autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Melanoma, cutaneous malignant 8 (CMM8) [MIM:614456]: A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. . Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the MiT/TFE family. / Contains 1 bHLH (basic helix-loop-helix) domain.

Protein post-translational modifications

Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. Phosphorylated at Ser-180 and Ser-516 following KIT signaling, trigerring a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome. / Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation.

Protein cellular localization

Nucleus

Research area

All research areas>Transcription Regulators>MITF
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize MITF (phospho Ser180) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!