Rabbit Polyclonal Phospho-MSK1 (S376) antibody (STJ90677)

$99.00$319.00

Reactivity: Human, Mouse
Applications: WB, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-MSK1 (S376) antibody (STJ90677)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for MSK1 (phospho Ser376) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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RPS6KA5 antibody, MSK1 antibody,|90 kDa ribosomal protein S6 kinase 5 antibody|EC 2.7.11.1 antibody|KS6A5_HUMAN antibody|MGC1911 antibody|Mitogen and stress activated protein kinase 1 antibody|MSPK1 antibody|Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody|Nuclear mitogen- and stress-activated protein kinase 1 antibody|Ribosomal protein S6 kinase 90kD polypeptide 5 antibody|Ribosomal protein S6 kinase 90kDa antibody|Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody|Ribosomal Protein S6 Kinase Alpha 5 antibody|Ribosomal protein S6 kinase alpha-5 antibody|RLPK antibody|RPS6KA5 antibody|RSK Like Protein Kinase antibody|RSK-like protein kinase antibody|RSKL antibody|S6K alpha 5 antibody|S6K-alpha-5 antibody|Phospho anti-MSK1 (S376) antibody [E375] (ab32190)
SCBT cat No: sc-130431|sc-9392|sc-25417|sc-9391|sc-130653|

 

Name

MSK1 (phospho Ser376) Polyclonal Antibody

Catalogue No.

STJ90677

Reactivity

Human, Mouse

Specificity

Phospho-MSK1 (S376) Polyclonal Antibody detects endogenous levels of MSK1 protein only when phosphorylated at S376.

Immunogen

Synthesized phospho-peptide derived from MSK1 (phospho Ser376) at AA range 310-390

Host

Rabbit

Applications

WB, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

90 kDa

Formulation

MSK1 (phospho Ser376) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

MSK1 (phospho Ser376) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Ribosomal protein S6 kinase alpha-5 antibody, S6K-alpha-5 antibody, 90 kDa ribosomal protein S6 kinase 5 antibody, Nuclear mitogen- and stress-activated protein kinase 1 antibody, RSK-like protein kinase antibody, RSKL antibody

Database links

Human UniProt/Swiss-Prot:O75582;Mouse UniPort/Swiss-Prot: Q8C050;Rat UniProt/Swiss-Port: D3ZY17;Human Entrez Gene: 9252;Mouse Entrez Gene: 73086;Rat Entrez Gene: Rn.8940

Protein names

Ribosomal protein S6 kinase alpha-5 , S6K-alpha-5 , 90 kDa ribosomal protein S6 kinase 5 , Nuclear mitogen- and stress-activated protein kinase 1 , RSK-like protein kinase , RSKL

Protein function

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at ‘Ser-276’ during oxidative stress. In erythropoietin-stimulated cells, is necessary for the ‘Ser-727’ phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at ‘Ser-191’ and ‘Ser-216’, and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of ‘Ser-1’ of histone H2A. Phosphorylates ‘Ser-10’ of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate ‘Ser-28’ of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury. / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Activated by phosphorylation at Ser-360, Thr-581 and Thr-700 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha, and by further autophosphorylation of Ser-212, Ser-376 and Ser-381 by the activated C-terminal kinase domain. The active N-terminal kinase domain finally phosphorylates downstream substrates, as well as Ser-750, Ser-752 and Ser-758 in its own C-terminal region.

Protein tissue specificity

Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

Protein sequence and domain

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. / Contains 1 AGC-kinase C-terminal domain. / Contains 2 protein kinase domains.

Protein post-translational modifications

Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-360, Thr-581 and Thr-700 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-750, Ser-752 and Ser-758 by the N-terminal kinase domain. / Ubiquitinated.

Protein cellular localization

Nucleus / Cytoplasm

Research area

All research areas>Kinases and Phosphatases>MSK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize MSK1 (phospho Ser376) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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