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Rabbit Polyclonal Phospho-MSK1 (T581) antibody (STJ90695)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;
Recommended protocols: check protocols
Click or hover above images to see image description for MSK1 (phospho Thr581) Polyclonal Antibody.
Check alternative names for the antibodyExpand
RPS6KA5 antibody, MSK1 antibody,|90 kDa ribosomal protein S6 kinase 5 antibody|EC 220.127.116.11 antibody|KS6A5_HUMAN antibody|MGC1911 antibody|Mitogen and stress activated protein kinase 1 antibody|MSPK1 antibody|Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody|Nuclear mitogen- and stress-activated protein kinase 1 antibody|Ribosomal protein S6 kinase 90kD polypeptide 5 antibody|Ribosomal protein S6 kinase 90kDa antibody|Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody|Ribosomal Protein S6 Kinase Alpha 5 antibody|Ribosomal protein S6 kinase alpha-5 antibody|RLPK antibody|RPS6KA5 antibody|RSK Like Protein Kinase antibody|RSK-like protein kinase antibody|RSKL antibody|S6K alpha 5 antibody|S6K-alpha-5 antibody|Phospho anti-MSK1 (S376) antibody [E375] (ab32190)
SCBT cat No: sc-130431|sc-9392|sc-25417|sc-9391|sc-130653|
MSK1 (phospho Thr581) Polyclonal Antibody
|Catalogue No.|| |
Phospho-MSK1 (T581) Polyclonal Antibody detects endogenous levels of MSK1 protein only when phosphorylated at T581.
Synthesized phospho-peptide derived from MSK1 (phospho Thr581) at AA range 520-600
WB, IHC, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;
|Molecular weight|| |
MSK1 (phospho Thr581) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
MSK1 (phospho Thr581) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Ribosomal protein S6 kinase alpha-5 antibody, S6K-alpha-5 antibody, 90 kDa ribosomal protein S6 kinase 5 antibody, Nuclear mitogen- and stress-activated protein kinase 1 antibody, RSK-like protein kinase antibody, RSKL antibody
|Protein names|| |
Ribosomal protein S6 kinase alpha-5 , S6K-alpha-5 , 90 kDa ribosomal protein S6 kinase 5 , Nuclear mitogen- and stress-activated protein kinase 1 , RSK-like protein kinase , RSKL
|Protein function|| |
Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at ‘Ser-276’ during oxidative stress. In erythropoietin-stimulated cells, is necessary for the ‘Ser-727’ phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at ‘Ser-191’ and ‘Ser-216’, and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of ‘Ser-1’ of histone H2A. Phosphorylates ‘Ser-10’ of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate ‘Ser-28’ of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury. / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Activated by phosphorylation at Ser-360, Thr-581 and Thr-700 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha, and by further autophosphorylation of Ser-212, Ser-376 and Ser-381 by the activated C-terminal kinase domain. The active N-terminal kinase domain finally phosphorylates downstream substrates, as well as Ser-750, Ser-752 and Ser-758 in its own C-terminal region.
|Protein tissue specificity|| |
Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. / Contains 1 AGC-kinase C-terminal domain. / Contains 2 protein kinase domains.
|Protein post-translational modifications|| |
Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-360, Thr-581 and Thr-700 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-750, Ser-752 and Ser-758 by the N-terminal kinase domain. / Ubiquitinated.
|Protein cellular localization|| |
Nucleus / Cytoplasm
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St John’s Laboratory Ltd.
|Product type|| |
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