Rabbit polyclonal Phospho-MTOR-S2448 antibody (AP0094)

$69.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal Phospho-MTOR-S2448 antibody (AP0094)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-MTOR-S2448 antibody.

Alternative names:

Check alternative names for the antibody

Expand

MTOR antibody,FLJ44809 antibody,FRAP antibody,FRAP1 antibody,FRAP2 antibody,RAFT1 antibody,RAPT1 antibody
dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody|FK506 binding protein 12 rapamycin associated protein 1 antibody|FK506 binding protein 12 rapamycin associated protein 2 antibody|FK506 binding protein 12 rapamycin complex associated protein 1 antibody|FK506-binding protein 12-rapamycin complex-associated protein 1 antibody|FKBP rapamycin associated protein antibody|FKBP12 rapamycin complex associated protein antibody|FKBP12-rapamycin complex-associated protein 1 antibody|FKBP12-rapamycin complex-associated protein antibody|FLJ44809 antibody|FRAP antibody|FRAP1 antibody|FRAP2 antibody|Mammalian target of rapamycin antibody|Mechanistic target of rapamycin antibody|mTOR antibody|MTOR_HUMAN antibody|OTTHUMP00000001983 antibody|RAFT1 antibody|Rapamycin and FKBP12 target 1 antibody|Rapamycin associated protein FRAP2 antibody|Rapamycin target protein 1 antibody|Rapamycin target protein antibody|RAPT1 antibody|Serine/threonine-protein kinase mTOR antibody|Anti-mTOR antibody (ab2732)

SCBT cat No: sc-136269|sc-293089|sc-1550|sc-8319|sc-1549|sc-135994|sc-33116|sc-86172|

Name

Rabbit polyclonal Phospho-MTOR-S2448 antibody

Catalogue No.

AP0094

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding serine 2448 of human mTOR/FRAP

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: /Observed: Refer to Figures

Formulation

Phospho-MTOR-S2448 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-MTOR-S2448 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MTOR antibody,FLJ44809 antibody,FRAP antibody,FRAP1 antibody,FRAP2 antibody,RAFT1 antibody,RAPT1 antibody

Database links

Human UniProt/Swiss-Prot:P42345

Protein names

MTOR,FLJ44809,FRAP,FRAP1,FRAP2,RAFT1,RAPT1

Protein function

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at ‘Ser-758’, disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at ‘Ser-473’ of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at ‘Ser-422’. Regulates osteoclastogensis by adjusting the expression of CEBPB isoforms (By similarity).

Protein tissue specificity

Expressed in numerous tissues, with highest levels in testis.

Protein sequence and domain

The kinase domain (PI3K/PI4K) is intrinsically active but has a highly restricted catalytic center.; The FAT domain forms three discontinuous subdomains of alpha-helical TPR repeats plus a single subdomain of HEAT repeats. The four domains pack sequentially to form a C-shaped a-solenoid that clamps onto the kinase domain .Belongs to the PI3/PI4-kinase family.; Contains 1 FAT domain.; Contains 1 FATC domain.; Contains 32 HEAT repeats.; Contains 1 PI3K/PI4K domain.; Contains 16 TPR repeats.

Protein post-translational modifications

Autophosphorylates when part of mTORC1 or mTORC2. Phosphorylation at Ser-1261, Ser-2159 and Thr-2164 promotes autophosphorylation. Phosphorylation in the kinase domain modulates the interactions of MTOR with RPTOR and PRAS40 and leads to increased intrinsic mTORC1 kinase activity. Phosphorylation at Thr-2173 in the ATP-binding region by AKT1 strongly reduces kinase activity.

Protein cellular localization

Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion outer membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome . Cytoplasm . Nucleus, PML body . Microsome membrane . Note: Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB .

Background

The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

Research area

All research areas>Kinases and Phosphatases>mTOR
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Phospho-MTOR-S2448 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!