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Rabbit Polyclonal Phospho-mTOR (T2446) antibody (STJ90544)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for mTOR (phospho Thr2446) Polyclonal Antibody.
Check alternative names for the antibodyExpand
MTOR antibody, FRAP antibody, FRAP1 antibody, FRAP2 antibody, RAFT1 antibody, RAPT1 antibody,|dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody|FK506 binding protein 12 rapamycin associated protein 1 antibody|FK506 binding protein 12 rapamycin associated protein 2 antibody|FK506 binding protein 12 rapamycin complex associated protein 1 antibody|FK506-binding protein 12-rapamycin complex-associated protein 1 antibody|FKBP rapamycin associated protein antibody|FKBP12 rapamycin complex associated protein antibody|FKBP12-rapamycin complex-associated protein 1 antibody|FKBP12-rapamycin complex-associated protein antibody|FLJ44809 antibody|FRAP antibody|FRAP1 antibody|FRAP2 antibody|Mammalian target of rapamycin antibody|Mechanistic target of rapamycin antibody|mTOR antibody|MTOR_HUMAN antibody|OTTHUMP00000001983 antibody|RAFT1 antibody|Rapamycin and FKBP12 target 1 antibody|Rapamycin associated protein FRAP2 antibody|Rapamycin target protein 1 antibody|Rapamycin target protein antibody|RAPT1 antibody|Serine/threonine-protein kinase mTOR antibody|Anti-mTOR antibody (ab2732)
SCBT cat No: sc-136269|sc-293089|sc-1550|sc-8319|sc-1549|sc-135994|sc-33116|sc-86172|
mTOR (phospho Thr2446) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-mTOR (T2446) Polyclonal Antibody detects endogenous levels of mTOR protein only when phosphorylated at T2446.
Synthesized phospho-peptide derived from mTOR (phospho Thr2446) at AA range 2390-2470
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000;
|Molecular weight|| |
mTOR (phospho Thr2446) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
mTOR (phospho Thr2446) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Serine/threonine-protein kinase mTOR antibody, FK506-binding protein 12-rapamycin complex-associated protein 1 antibody, FKBP12-rapamycin complex-associated protein antibody, Mammalian target of rapamycin antibody, mTOR antibody, Mechanistic target of rapamycin antibody, Rapamycin and FKBP12 target 1 antibody, Rapamycin target protein 1 antibody
|Protein names|| |
Serine/threonine-protein kinase mTOR , FK506-binding protein 12-rapamycin complex-associated protein 1 , FKBP12-rapamycin complex-associated protein , Mammalian target of rapamycin , mTOR , Mechanistic target of rapamycin , Rapamycin and FKBP12 target 1 , Rapamycin target protein 1
|Protein function|| |
Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at ‘Ser-758’, disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at ‘Ser-473’ of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at ‘Ser-422’. Regulates osteoclastogensis by adjusting the expression of CEBPB isoforms (By similarity). / ATP + a protein = ADP + a phosphoprotein. / Activation of mTORC1 by growth factors such as insulin involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase a potent activator of the protein kinase activity of mTORC1. Insulin-stimulated and amino acid-dependent phosphorylation at Ser-1261 promotes autophosphorylation and the activation of mTORC1. Activation by amino acids requires relocalization of the mTORC1 complex to lysosomes that is mediated by the Ragulator complex, SLC38A9, and the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD . On the other hand, low cellular energy levels can inhibit mTORC1 through activation of PRKAA1 while hypoxia inhibits mTORC1 through a REDD1-dependent mechanism which may also require PRKAA1. The kinase activity of MTOR within the mTORC1 complex is positively regulated by MLST8 and negatively regulated by DEPTOR and AKT1S1. MTOR phosphorylates RPTOR which in turn inhibits mTORC1. MTOR is the target of the immunosuppressive and anti-cancer drug rapamycin which acts in complex with FKBP1A/FKBP12, and specifically inhibits its kinase activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. It may be regulated by RHEB but in an indirect manner through the PI3K signaling pathway.
|Protein tissue specificity|| |
Expressed in numerous tissues, with highest levels in testis.
|Involvement in disease|| |
Smith-Kingsmore syndrome (SKS) [MIM:616638]: An autosomal dominant syndrome characterized by intellectual disability, macrocephaly, seizures, umbilical hernia, and facial dysmorphic features. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
The kinase domain (PI3K/PI4K) is intrinsically active but has a highly restricted catalytic center. / The FAT domain forms three discontinuous subdomains of alpha-helical TPR repeats plus a single subdomain of HEAT repeats. The four domains pack sequentially to form a C-shaped a-solenoid that clamps onto the kinase domain (PubMed:23636326). / Belongs to the PI3/PI4-kinase family. / Contains 1 FAT domain. / Contains 1 FATC domain. / Contains 32 HEAT repeats. / Contains 1 PI3K/PI4K domain. / Contains 16 TPR repeats.
|Protein post-translational modifications|| |
Autophosphorylates when part of mTORC1 or mTORC2. Phosphorylation at Ser-1261, Ser-2159 and Thr-2164 promotes autophosphorylation. Phosphorylation in the kinase domain modulates the interactions of MTOR with RPTOR and PRAS40 and leads to increased intrinsic mTORC1 kinase activity. Phosphorylation at Thr-2173 in the ATP-binding region by AKT1 strongly reduces kinase activity.
|Protein cellular localization|| |
Endoplasmic reticulum membrane / Peripheral membrane protein / Cytoplasmic side / Golgi apparatus membrane / Peripheral membrane protein / Cytoplasmic side / Mitochondrion outer membrane / Peripheral membrane protein / Cytoplasmic side / Lysosome / Cytoplasm / Nucleus > PML body / Microsome membrane
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St John’s Laboratory Ltd.
|Product type|| |
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