Rabbit Polyclonal Phospho-MyoD (S200) antibody (STJ90472)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-MyoD (S200) antibody (STJ90472)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for MyoD (phospho Ser200) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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MYOD1 antibody, BHLHC1 antibody, MYF3 antibody, MYOD antibody,|bHLHc1 antibody|Class C basic helix-loop-helix protein 1 antibody|MYF 3 antibody|Myf-3 antibody|MYF3 antibody|Myoblast determination protein 1 antibody|Myod 1 antibody|MYOD antibody|MYOD1 antibody|MYOD1_HUMAN antibody|Myogenic differentiation 1 antibody|Myogenic factor 3 antibody|Myogenic factor MYF 3 antibody|Myogenin D1 antibody|PUM antibody|Anti-MyoD1 antibody [5.2F] (ab16148)
SCBT cat No: sc-377186|

 

Name

MyoD (phospho Ser200) Polyclonal Antibody

Catalogue No.

STJ90472

Reactivity

Human, Mouse, Rat

Specificity

Phospho-MyoD (S200) Polyclonal Antibody detects endogenous levels of MyoD protein only when phosphorylated at S200.

Immunogen

Synthesized phospho-peptide derived from MyoD (phospho Ser200) at AA range 140-220

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

34 kDa

Formulation

MyoD (phospho Ser200) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

MyoD (phospho Ser200) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Myoblast determination protein 1 antibody, Class C basic helix-loop-helix protein 1 antibody, bHLHc1 antibody, Myogenic factor 3 antibody, Myf-3 antibody

Database links

Human UniProt/Swiss-Prot:P15172;Mouse UniPort/Swiss-Prot: P10085;Rat UniProt/Swiss-Port: Q02346;Human Entrez Gene: 4654;Mouse Entrez Gene: 17927;Rat Entrez Gene: Rn.9493

Protein names

Myoblast determination protein 1 , Class C basic helix-loop-helix protein 1 , bHLHc1 , Myogenic factor 3 , Myf-3

Protein function

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).

Protein sequence and domain

Contains 1 bHLH (basic helix-loop-helix) domain.

Protein post-translational modifications

Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. / Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity). / Ubiquitinated on the N-terminus; which is required for proteasomal degradation. / Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.

Protein cellular localization

Nucleus

Research area

All research areas>Transcription Regulators>MyoD
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize MyoD (phospho Ser200) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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