Rabbit Polyclonal Phospho-MYPT1 (T853) antibody (STJ90822)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-MYPT1 (T853) antibody (STJ90822)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for MYPT1 (phospho Thr853) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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PPP1R12A antibody, MBS antibody, MYPT1 antibody,|Myosin phosphatase target subunit 1 antibody|Myosin phosphatase target subunit 2 antibody|Myosin phosphatase targeting subunit 1 antibody|Myosin phosphatase targeting subunit 2 antibody|MYPT1 antibody|MYPT2 antibody|PPP1R12A antibody|PPP1R12B antibody|Protein phosphatase 1 regulatory inhibitor subunit 12A antibody|Protein phosphatase 1 regulatory Inhibitor subunit 12B antibody|Protein phosphatase 1 regulatory subunit 12A antibody|Protein phosphatase 1 regulatory subunit 12B antibody|Protein phosphatase myosin binding subunit antibody|Anti-Myosin Phosphatase 1+Myosin Phosphatase 2 antibody [YE336] (ab32519)
SCBT cat No: sc-292988|

Name

MYPT1 (phospho Thr853) Polyclonal Antibody

Catalogue No.

STJ90822

Reactivity

Human, Mouse, Rat

Specificity

Phospho-MYPT1 (T853) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.

Immunogen

Synthesized phospho-peptide derived from MYPT1 (phospho Thr853) at AA range 590-670

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

130 kDa

Formulation

MYPT1 (phospho Thr853) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

MYPT1 (phospho Thr853) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Protein phosphatase 1 regulatory subunit 12A antibody, Myosin phosphatase-targeting subunit 1 antibody, Myosin phosphatase target subunit 1 antibody, Protein phosphatase myosin-binding subunit antibody

Database links

Human UniProt/Swiss-Prot:O14974;Mouse UniPort/Swiss-Prot: Q9DBR7;Rat UniProt/Swiss-Port: Q10728;Human Entrez Gene: 4659;Mouse Entrez Gene: 17931;Rat Entrez Gene: Rn.162937

Protein names

Protein phosphatase 1 regulatory subunit 12A , Myosin phosphatase-targeting subunit 1 , Myosin phosphatase target subunit 1 , Protein phosphatase myosin-binding subunit

Protein function

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Protein tissue specificity

Expressed in striated muscles, specifically in type 2a fibers (at protein level).

Protein sequence and domain

Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase. / The KVKF motif mediates interaction with PPP1CB. / Contains 6 ANK repeats.

Protein post-translational modifications

Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK; may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A.

Protein cellular localization

Cytoplasm

Research area

All research areas>Signaling Intermediates>MYPT1
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize MYPT1 (phospho Thr853) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human lung tissue

1: MYPT1 (phospho Thr853) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung tissue

1: MYPT1 (phospho Thr853) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat heart tissue

1: MYPT1 (phospho Thr853) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat heart tissue

1: MYPT1 (phospho Thr853) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat kidney tissue

1: MYPT1 (phospho Thr853) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat kidney tissue

1: MYPT1 (phospho Thr853) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.


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