Rabbit Polyclonal Phospho-Nek9 (T210) antibody (STJ90724)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Nek9 (T210) antibody (STJ90724)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Nek9 (phospho Thr210) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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NEK9 antibody, KIAA1995 antibody, NEK8 antibody, NERCC antibody,|KIAA1995 antibody|Serine/threonine protein kinase Nek9 antibody|Serine/threonine-protein kinase Nek9 antibody|Anti-NEK9 antibody [EP7361] (ab138488)
SCBT cat No: sc-100401|sc-50763|sc-50765|

 

Name

Nek9 (phospho Thr210) Polyclonal Antibody

Catalogue No.

STJ90724

Reactivity

Human, Mouse, Rat

Specificity

Phospho-Nek9 (T210) Polyclonal Antibody detects endogenous levels of Nek9 protein only when phosphorylated at T210.

Immunogen

Synthesized phospho-peptide derived from Nek9 (phospho Thr210) at AA range 150-230

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

130 kDa

Formulation

Nek9 (phospho Thr210) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Nek9 (phospho Thr210) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Serine/threonine-protein kinase Nek9 antibody, Nercc1 kinase antibody, Never in mitosis A-related kinase 9 antibody, NimA-related protein kinase 9 antibody, NimA-related kinase 8 antibody, Nek8 antibody

Database links

Human UniProt/Swiss-Prot:Q8TD19;Mouse UniPort/Swiss-Prot: Q8K1R7;Human Entrez Gene: 91754;Mouse Entrez Gene: 217718;Rat Entrez Gene: Rn.102369

Protein names

Serine/threonine-protein kinase Nek9 , Nercc1 kinase , Never in mitosis A-related kinase 9 , NimA-related protein kinase 9 , NimA-related kinase 8 , Nek8

Protein function

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively. / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions. Sensitive to increasing concentration of detergents. It is not cell-cycle regulated but activity is higher in G0-arrested cells.

Protein tissue specificity

Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.

Protein sequence and domain

Dimerizes through its coiled-coil domain. / Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. / Contains 1 protein kinase domain. / Contains 6 RCC1 repeats.

Protein post-translational modifications

Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Kinases and Phosphatases>Nek
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Nek9 (phospho Thr210) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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