Rabbit Polyclonal Phospho-NF2 (S518) antibody (STJ90340)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-NF2 (S518) antibody (STJ90340)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for NF2 (phospho Ser518) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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NF2 antibody, SCH antibody,|ACN antibody|BANF antibody|Bilateral acoustic neuroma antibody|MERL_HUMAN antibody|Merlin antibody|Moesin ezrin radixin like protein antibody|Moesin ezrin radizin like antibody|Moesin-ezrin-radixin-like protein antibody|Neurofibromatosis 2 antibody|Neurofibromatosis type 2 antibody|Neurofibromatosis2 antibody|Neurofibromin 2 antibody|Neurofibromin-2 antibody|Neurofibromin2 antibody|NF 2 antibody|Nf2 antibody|SCH antibody|Schwannomerlin antibody|Schwannomin antibody|Anti-NF2 / Merlin antibody [AF1G4] (ab88957)
SCBT cat No: sc-331|sc-55575|sc-332|sc-55574|sc-28247|sc-271718|

 

Name

NF2 (phospho Ser518) Polyclonal Antibody

Catalogue No.

STJ90340

Reactivity

Human, Mouse, Rat

Specificity

Phospho-NF2 (S518) Polyclonal Antibody detects endogenous levels of NF2 protein only when phosphorylated at S518.

Immunogen

Synthesized phospho-peptide derived from NF2 (phospho Ser518) at AA range 460-540

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

70 kDa

Formulation

NF2 (phospho Ser518) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

NF2 (phospho Ser518) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Merlin antibody, Moesin-ezrin-radixin-like protein antibody, Neurofibromin-2 antibody, Schwannomerlin antibody, Schwannomin antibody

Database links

Human UniProt/Swiss-Prot:P35240;Mouse UniPort/Swiss-Prot: P46662;Rat UniProt/Swiss-Port: Q63648;Human Entrez Gene: 4771;Mouse Entrez Gene: 18016;Rat Entrez Gene: Rn.46695

Protein names

Merlin , Moesin-ezrin-radixin-like protein , Neurofibromin-2 , Schwannomerlin , Schwannomin

Protein function

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.

Protein tissue specificity

Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.

Involvement in disease

Neurofibromatosis 2 (NF2) [MIM:101000]: Genetic disorder characterized by bilateral vestibular schwannomas (formerly called acoustic neuromas), schwannomas of other cranial and peripheral nerves, meningiomas, and ependymomas. It is inherited in an autosomal dominant fashion with full penetrance. Affected individuals generally develop symptoms of eighth-nerve dysfunction in early adulthood, including deafness and balance disorder. Although the tumors of NF2 are histologically benign, their anatomic location makes management difficult, and patients suffer great morbidity and mortality. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Schwannomatosis 1 (SWNTS1) [MIM:162091]: A cancer syndrome in which patients develop multiple non-vestibular schwannomas, benign neoplasms that arise from Schwann cells of the cranial, peripheral, and autonomic nerves. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Mesothelioma, malignant (MESOM) [MIM:156240]: An aggressive neoplasm of the serosal lining of the chest. It appears as broad sheets of cells, with some regions containing spindle-shaped, sarcoma-like cells and other regions showing adenomatous patterns. Pleural mesotheliomas have been linked to exposure to asbestos. . Note: The disease may be caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Contains 1 FERM domain.

Protein post-translational modifications

Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail. / Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation.

Protein cellular localization

Cell projection > filopodium membrane; Peripheral membrane protein; Cytoplasmic side / Cell projection > ruffle membrane; Peripheral membrane protein; Cytoplasmic side / Nucleus / Cytoplasm > perinuclear region / Cytoplasmic granule / Cytoplasm > perinuclear region / Cytoplasmic granule / Nucleus / Cell projection > filopodium membrane; Peripheral membrane protein; Cytoplasmic side / Cell projection > ruffle membrane; Peripheral membrane protein; Cytoplasmic side / Cytoplasm > perinuclear region / Cytoplasmic granule / Cytoplasm > cytoskeleton

Research area

All research areas>Tumor Suppressor/Apoptosis>NF2
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize NF2 (phospho Ser518) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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