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Rabbit Polyclonal Phospho-NFkappaB-p100 (S872) antibody (STJ90535)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:20000;
Recommended protocols: check protocols
Click or hover above images to see image description for NFkappaB-p100 (phospho Ser872) Polyclonal Antibody.
Check alternative names for the antibodyExpand
NFKB2 antibody, LYT10 antibody,|CVID10 antibody|DNA binding factor KBF2 antibody|H2TF1 antibody|Lymphocyte translocation chromosome 10 protein antibody|LYT 10 antibody|NF kB2 antibody|NFKB p52/p100 subunit antibody|Nuclear factor Kappa B subunit 2 antibody|Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) antibody|Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody|Oncogene Lyt 10 antibody|p100 antibody|Transcription factor NFKB2 antibody|Anti-NFkB p100 antibody [EPR18756] (ab191594)
SCBT cat No: sc-848|sc-7386|sc-298|sc-56735|sc-71675|
NFkappaB-p100 (phospho Ser872) Polyclonal Antibody
|Catalogue No.|| |
Phospho-NFkappaB-p100 (S872) Polyclonal Antibody detects endogenous levels of NFkappaB-p100 protein only when phosphorylated at S872.
Synthesized phospho-peptide derived from NFkappaB-p100 (phospho Ser872) at AA range 810-890
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:20000;
|Molecular weight|| |
NFkappaB-p100 (phospho Ser872) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
NFkappaB-p100 (phospho Ser872) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Nuclear factor NF-kappa-B p100 subunit antibody, DNA-binding factor KBF2 antibody, H2TF1 antibody, Lymphocyte translocation chromosome 10 protein antibody, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 antibody, Oncogene Lyt-10 antibody, Lyt10 antibody
|Protein names|| |
Nuclear factor NF-kappa-B p100 subunit , DNA-binding factor KBF2 , H2TF1 , Lymphocyte translocation chromosome 10 protein , Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 , Oncogene Lyt-10 , Lyt10
|Protein function|| |
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5′-GGRNNYYCC-3′, located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
|Involvement in disease|| |
Note: A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10;14)(q24;q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant.; Note: A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which codes for a truncated 80 kDa protein (p80HT).; Note: In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions.; Immunodeficiency, common variable, 10 (CVID10) [MIM:615577]: A primary immunodeficiency characterized by childhood-onset of recurrent infections, hypogammaglobulinemia, and decreased numbers of memory and marginal zone B-cells. Some patients may develop autoimmune features and have circulating autoantibodies. An unusual feature is central adrenal insufficiency. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
The C-terminus of p100 might be involved in cytoplasmic retention, inhibition of DNA-binding by p52 homodimers, and/or transcription activation. / The glycine-rich region (GRR) appears to be a critical element in the generation of p52. / Contains 7 ANK repeats. / Contains 1 death domain. / Contains 1 RHD (Rel-like) domain.
|Protein post-translational modifications|| |
While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing. / Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing. / Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which contains the death domain.
|Protein cellular localization|| |
Nucleus / Cytoplasm
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St John’s Laboratory Ltd.
|Product type|| |
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