Rabbit Polyclonal Phospho-NOS3 (S615) antibody (STJ90541)


Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-NOS3 (S615) antibody (STJ90541)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:40000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for NOS3 (phospho Ser615) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


NOS3 antibody,|cNOS antibody|Constitutive NOS antibody|EC NOS antibody|EC-NOS antibody|ecNOS antibody|Endothelial nitric oxidase synthase antibody|Endothelial nitric oxide synthase antibody|Endothelial nitric oxide synthase 3 antibody|Endothelial NOS antibody|eNOS antibody|Nitric oxide synthase 3 (endothelial cell) antibody|Nitric oxide synthase 3 antibody|Nitric oxide synthase 3 endothelial cell antibody|Nitric oxide synthase endothelial antibody|Nitric oxide synthase, endothelial antibody|NOS 3 antibody|NOS III antibody|NOS type III antibody|NOS3 antibody|NOS3_HUMAN antibody|NOSIII antibody|Anti-eNOS antibody [M221] (ab76198)
SCBT cat No: sc-376751|sc-136977|sc-654|sc-376542|sc-8311|sc-653|sc-137118|sc-46423|sc-365363|sc-137111|sc-67003|sc-137117|sc-373954|



NOS3 (phospho Ser615) Polyclonal Antibody

Catalogue No.



Human, Mouse, Rat


Phospho-NOS3 (S615) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at S615.


Synthesized phospho-peptide derived from NOS3 (phospho Ser615) at AA range 550-630





Recommended dilution

WB 1:500-1:2000; ELISA 1:40000;







Molecular weight

130 kDa


NOS3 (phospho Ser615) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


NOS3 (phospho Ser615) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Nitric oxide synthase, endothelial antibody, Constitutive NOS antibody, cNOS antibody, EC-NOS antibody, Endothelial NOS antibody, eNOS antibody, NOS type III antibody, NOSIII antibody

Database links

Human UniProt/Swiss-Prot:P29474;Mouse UniPort/Swiss-Prot: P70313;Rat UniProt/Swiss-Port: Q62600;Human Entrez Gene: 4846;Mouse Entrez Gene: 18127;Rat Entrez Gene: Rn.44265

Protein names

Nitric oxide synthase, endothelial , Constitutive NOS , cNOS , EC-NOS , Endothelial NOS , eNOS , NOS type III , NOSIII

Protein function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. / Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. / 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. / heme / FAD / FMN / 5,6,7,8-tetrahydrobiopterin / Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein tissue specificity

Platelets, placenta, liver and kidney.

Involvement in disease

Note: Variation in NOS3 seem to be associated with susceptibility to coronary spasm. .

Protein sequence and domain

Belongs to the NOS family. / Contains 1 FAD-binding FR-type domain. / Contains 1 flavodoxin-like domain.

Protein post-translational modifications

Phosphorylation by AMPK at Ser-1177 in the presence of Ca2+-calmodulin (CaM) activates activity. In absence of Ca2+-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.

Protein cellular localization

Cell membrane / Membrane > caveola / Cytoplasm > cytoskeleton / Golgi apparatus

Research area

All research areas>Synthesis and Degradation>NOS3
(View all antibody categories related to Synthesis and Degradation)


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St John’s Laboratory Ltd.

Product type

Primary antibody


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