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Rabbit Polyclonal Phospho-p38 (Y182) antibody (STJ90363)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for p38 (phospho Tyr182) Polyclonal Antibody.
Check alternative names for the antibodyExpand
MAPK14 antibody, CSBP antibody, CSBP1 antibody, CSBP2 antibody, CSPB1 antibody, MXI2 antibody, SAPK2A antibody,|CSAID Binding Protein 1 antibody|CSAID binding protein antibody|CSAID-binding protein antibody|Csaids binding protein antibody|CSBP 1 antibody|CSBP 2 antibody|CSBP antibody|CSBP1 antibody|CSBP2 antibody|CSPB1 antibody|Cytokine suppressive anti-inflammatory drug-binding protein antibody|EXIP antibody|MAP kinase 14 antibody|MAP kinase MXI2 antibody|MAP kinase p38 alpha antibody|MAPK 14 antibody|MAPK14 antibody|MAX interacting protein 2 antibody|MAX-interacting protein 2 antibody|Mitogen Activated Protein Kinase 14 antibody|Mitogen activated protein kinase p38 alpha antibody|Mitogen-activated protein kinase 14 antibody|Mitogen-activated protein kinase p38 alpha antibody|MK14_HUMAN antibody|Mxi 2 antibody|MXI2 antibody|p38 ALPHA antibody|p38 antibody|p38 MAP kinase antibody|p38 MAPK antibody|p38 mitogen activated protein kinase antibody|p38ALPHA antibody|p38alpha Exip antibody|PRKM14 antibody|PRKM15 antibody|RK antibody|SAPK2A antibody|Anti-p38 antibody [E229] (ab170099)
SCBT cat No: sc-136210|sc-81621|sc-33688|sc-535|sc-398305|sc-271120|sc-166357|sc-728|sc-7972|sc-7149|sc-101427|sc-33689|sc-6176|sc-6187|
p38 (phospho Tyr182) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-p38 (Y182) Polyclonal Antibody detects endogenous levels of p38 protein only when phosphorylated at Y182.
Synthesized phospho-peptide derived from p38 (phospho Tyr182) at AA range 120-200
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
|Molecular weight|| |
p38 (phospho Tyr182) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
p38 (phospho Tyr182) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Mitogen-activated protein kinase 14 antibody, MAP kinase 14 antibody, MAPK 14 antibody, Cytokine suppressive anti-inflammatory drug-binding protein antibody, CSAID-binding protein antibody, CSBP antibody, MAP kinase MXI2 antibody, MAX-interacting protein 2 antibody, Mitogen-activated protein kinase p38 alpha antibody, MAP kinase p38 alpha antibody, Stress-activated protein kinase 2a antibody, SAPK2a antibody
|Protein names|| |
Mitogen-activated protein kinase 14 , MAP kinase 14 , MAPK 14 , Cytokine suppressive anti-inflammatory drug-binding protein , CSAID-binding protein , CSBP , MAP kinase MXI2 , MAX-interacting protein 2 , Mitogen-activated protein kinase p38 alpha , MAP kinase p38 alpha , Stress-activated protein kinase 2a , SAPK2a
|Protein function|| |
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on ‘Ser-10’ (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at ‘Thr-113’. / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as bacterial lipopolysaccharide (LPS) and interleukin-1. Activation occurs through dual phosphorylation of Thr-180 and Tyr-182 by either of two dual specificity kinases, MAP2K3/MKK3 or MAP2K6/MKK6, and potentially also MAP2K4/MKK4, as well as by TAB1-mediated autophosphorylation. MAPK14 phosphorylated on both Thr-180 and Tyr-182 is 10-20-fold more active than MAPK14 phosphorylated only on Thr-180, whereas MAPK14 phosphorylated on Tyr-182 alone is inactive. whereas Thr-180 is necessary for catalysis, Tyr-182 may be required for auto-activation and substrate recognition. Phosphorylated at Tyr-323 by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells. This alternative pathway is inhibited by GADD45A. Inhibited by dual specificity phosphatases, such as DUSP1, DUSP10, and DUSP16. Specifically inhibited by the binding of pyridinyl-imidazole compounds, which are cytokine-suppressive anti-inflammatory drugs (CSAID). Isoform Mxi2 is 100-fold less sensitive to these agents than the other isoforms and is not inhibited by DUSP1. Isoform Exip is not activated by MAP2K6. SB203580 is an inhibitor of MAPK14.
|Protein tissue specificity|| |
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
|Protein sequence and domain|| |
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. / Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Dually phosphorylated on Thr-180 and Tyr-182 by the MAP2Ks MAP2K3/MKK3, MAP2K4/MKK4 and MAP2K6/MKK6 in response to inflammatory citokines, environmental stress or growth factors, which activates the enzyme. Dual phosphorylation can also be mediated by TAB1-mediated autophosphorylation. TCR engagement in T-cells also leads to Tyr-323 phosphorylation by ZAP70. Dephosphorylated and inactivated by DUPS1, DUSP10 and DUSP16. PPM1D also mediates dephosphorylation and inactivation of MAPK14 . / Acetylated at Lys-53 and Lys-152 by KAT2B and EP300. Acetylation at Lys-53 increases the affinity for ATP and enhances kinase activity. Lys-53 and Lys-152 are deacetylated by HDAC3. / Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway.
|Protein cellular localization|| |
Cytoplasm / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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