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Rabbit Polyclonal Phospho-p70 S6 kinase alpha (S371) antibody (STJ90824)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
RPS6KB1 antibody, STK14A antibody,|70 kDa ribosomal protein S6 kinase 1 antibody|KS6B1_HUMAN antibody|p70 alpha antibody|P70 beta 1 antibody|p70 ribosomal S6 kinase alpha antibody|p70 ribosomal S6 kinase beta 1 antibody|p70 S6 kinase alpha antibody|P70 S6 Kinase antibody|p70 S6 kinase, alpha 1 antibody|p70 S6 kinase, alpha 2 antibody|p70 S6K antibody|p70 S6K-alpha antibody|p70 S6KA antibody|p70(S6K) alpha antibody|p70(S6K)-alpha antibody|p70-alpha antibody|p70-S6K 1 antibody|p70-S6K antibody|P70S6K antibody|P70S6K1 antibody|p70S6Kb antibody|PS6K antibody|Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody|Ribosomal protein S6 kinase beta 1 antibody|Ribosomal protein S6 kinase beta-1 antibody|Ribosomal protein S6 kinase I antibody|RPS6KB1 antibody|S6K antibody|S6K-beta-1 antibody|S6K1 antibody|Serine/threonine kinase 14 alpha antibody|Serine/threonine-protein kinase 14A antibody|STK14A antibody|Anti-S6K1 antibody [E343] (ab32529)
SCBT cat No: sc-393967|sc-230|sc-514501|sc-9027|sc-8418|sc-293269|sc-9381|sc-9379|sc-130833|sc-56190|sc-56191|
p70 S6 kinase alpha (phospho Ser371) Polyclonal Antibody
|Catalogue No.|| |
Phospho-p70 S6 kinase alpha (S371) Polyclonal Antibody detects endogenous levels of p70 S6 kinase alpha protein only when phosphorylated at S371.
Synthesized phospho-peptide derived from p70 S6 kinase alpha (phospho Ser371) at AA range 310-390
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;
|Molecular weight|| |
p70 S6 kinase alpha (phospho Ser371) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
p70 S6 kinase alpha (phospho Ser371) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Ribosomal protein S6 kinase beta-1 antibody, S6K-beta-1 antibody, S6K1 antibody, 70 kDa ribosomal protein S6 kinase 1 antibody, P70S6K1 antibody, p70-S6K 1 antibody, Ribosomal protein S6 kinase I antibody, Serine/threonine-protein kinase 14A antibody, p70 ribosomal S6 kinase alpha antibody, p70 S6 kinase alpha antibody, p70 S6K-alpha antibody, p70 S6KA antibody
|Protein names|| |
Ribosomal protein S6 kinase beta-1 , S6K-beta-1 , S6K1 , 70 kDa ribosomal protein S6 kinase 1 , P70S6K1 , p70-S6K 1 , Ribosomal protein S6 kinase I , Serine/threonine-protein kinase 14A , p70 ribosomal S6 kinase alpha , p70 S6 kinase alpha , p70 S6K-alpha , p70 S6KA
|Protein function|| |
Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR. / ATP + a protein = ADP + a phosphoprotein. / Activation requires multiple phosphorylation events on serine/threonine residues. Activation appears to be first mediated by phosphorylation of multiple sites in the autoinhibitory domain, which facilitates phosphorylation at Thr-412, disrupting the autoinhibitory mechanism and allowing phosphorylation of Thr-252 by PDPK1. The active conformation of the kinase is believed to be stabilized by a mechanism involving three conserved phosphorylation sites located in the kinase domain activation loop (Thr-252) and in the AGC-kinase C-terminal domain (Ser-394 in the middle of the tail/linker region and Thr-412 within a hydrophobic motif at its end). Activated by mTORC1; isoform Alpha I and isoform Alpha II are sensitive to rapamycin, which inhibits activating phosphorylation at Thr-412. Activated by PDPK1.
|Protein tissue specificity|| |
|Protein sequence and domain|| |
The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop. / The TOS (TOR signaling) motif is essential for activation by mTORC1. / Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. / Contains 1 AGC-kinase C-terminal domain. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.
|Protein cellular localization|| |
Cell junction > synapse > synaptosome / Mitochondrion outer membrane / Mitochondrion / Nucleus / Cytoplasm / Cytoplasm
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St John’s Laboratory Ltd.
|Product type|| |
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