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Rabbit Polyclonal Phospho-PAKalpha/beta/gamma (S144/141/139) antibody (STJ90827)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for PAKalpha/beta/gamma (phospho Ser144/141/139) Polyclonal Antibody.
Check alternative names for the antibodyExpand
PAK1 antibody,|ADRB2 antibody|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody|p65 PAK antibody|p65-PAK antibody|P68-PAK antibody|PAK alpha antibody|PAK-1 antibody|Pak1 antibody|PAK1_HUMAN antibody|Paka antibody|PAKalpha antibody|Protein kinase MUK2 antibody|Rac/p21-activated kinase antibody|Serine/threonine-protein kinase PAK 1 antibody|STE20 homolog yeast antibody|Anti-PAK1 antibody [EP795Y] (ab40852)
SCBT cat No: sc-882|sc-50693|sc-50694|sc-293362|sc-10925|sc-10927|sc-161294|
PAKalpha/beta/gamma (phospho Ser144/141/139) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-PAKalpha/beta/gamma (S144/141/139) Polyclonal Antibody detects endogenous levels of PAKalpha/beta/gamma protein only when phosphorylated at S144/141/139.
Synthesized phospho-peptide derived from PAKalpha/beta/gamma (phospho Ser144/141/139) at AA range 80-160
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
|Molecular weight|| |
PAKalpha/beta/gamma (phospho Ser144/141/139) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PAKalpha/beta/gamma (phospho Ser144/141/139) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Serine/threonine-protein kinase PAK 1 antibody, Alpha-PAK antibody, p21-activated kinase 1 antibody, PAK-1 antibody, p65-PAK antibody
|Protein names|| |
Serine/threonine-protein kinase PAK 1 , Alpha-PAK , p21-activated kinase 1 , PAK-1 , p65-PAK
|Protein function|| |
Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at ‘Ser-338’ and ‘Ser-339’ resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at ‘Ser-246’ promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion . / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Phosphorylation of Thr-84 by OXSR1 inhibits activation (By similarity). Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, and enables activation by phosphorylation of Thr-423.
|Protein tissue specificity|| |
Overexpressed in gastric cancer cells and tissues (at protein level) (PubMed:25766321).
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. / Contains 1 CRIB domain. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-423 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-423 by BRSK2 and by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites.
|Protein cellular localization|| |
Cytoplasm / Cell junction > focal adhesion / Cell membrane / Cell projection > ruffle membrane / Cell projection > invadopodium
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St John’s Laboratory Ltd.
|Product type|| |
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