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Rabbit Polyclonal Phospho-PDGFR-alpha (Y849) antibody (STJ90601)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for PDGFR-alpha (phospho Tyr849) Polyclonal Antibody.
Check alternative names for the antibodyExpand
PDGFRA antibody, PDGFR2 antibody, RHEPDGFRA antibody,|Alpha-type platelet-derived growth factor receptor antibody|CD140 antigen-like family member A antibody|CD140a antibody|CD140a antigen antibody|MGC74795 antibody|PDGF alpha chain antibody|PDGF-R-alpha antibody|PDGFR 2 antibody|PDGFR alpha antibody|PDGFR2 antibody|PDGFRA antibody|PDGFRA/BCR fusion antibody|PGFRA_HUMAN antibody|Platelet derived growth factor receptor 2 antibody|Platelet derived growth factor receptor alpha antibody|Platelet derived growth factor receptor alpha polypeptide antibody|Platelet derived growth factor receptor antibody|Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody|RHEPDGFRA antibody|Phospho anti-PDGF Receptor alpha (Y754) antibody (ab5460)
SCBT cat No: sc-101569|
PDGFR-alpha (phospho Tyr849) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-PDGFR-alpha (Y849) Polyclonal Antibody detects endogenous levels of PDGFR-alpha protein only when phosphorylated at Y849.
Synthesized phospho-peptide derived from PDGFR-alpha (phospho Tyr849) at AA range 790-870
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
|Molecular weight|| |
PDGFR-alpha (phospho Tyr849) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PDGFR-alpha (phospho Tyr849) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Platelet-derived growth factor receptor alpha antibody, PDGF-R-alpha antibody, PDGFR-alpha antibody, Alpha platelet-derived growth factor receptor antibody, Alpha-type platelet-derived growth factor receptor antibody, CD140 antigen-like family member A antibody, CD140a antigen antibody, Platelet-derived growth factor alpha receptor antibody, Platelet-derived growth factor receptor 2 antibody, PDGFR-2 antibody, CD antigen CD140a antibody
|Protein names|| |
Platelet-derived growth factor receptor alpha , PDGF-R-alpha , PDGFR-alpha , Alpha platelet-derived growth factor receptor , Alpha-type platelet-derived growth factor receptor , CD140 antigen-like family member A , CD140a antigen , Platelet-derived growth factor alpha receptor , Platelet-derived growth factor receptor 2 , PDGFR-2 , CD antigen CD140a
|Protein function|| |
Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands – homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca2+ and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Present in an inactive conformation in the absence of bound ligand. Binding of PDGFA and/or PDGFB leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by imatinib, nilotinib and sorafenib.
|Protein tissue specificity|| |
Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
|Involvement in disease|| |
Note: A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). Mutations that cause overexpression and/or constitutive activation of PDGFRA may be a cause of hypereosinophilic syndrome. .; Gastrointestinal stromal tumor (GIST) [MIM:606764]: Common mesenchymal neoplasms arising in the gastrointestinal tract, most often in the stomach. They are histologically, immunohistochemically, and genetically different from typical leiomyomas, leiomyosarcomas, and schwannomas. Most GISTs are composed of a fairly uniform population of spindle-shaped cells. Some tumors are dominated by epithelioid cells or contain a mixture of spindle and epithelioid morphologies. Primary GISTs in the gastrointestinal tract commonly metastasize in the omentum and mesenteries, often as multiple nodules. However, primary tumors may also occur outside of the gastrointestinal tract, in other intra-abdominal locations, especially in the omentum and mesentery. . Note: The gene represented in this entry may be involved in disease pathogenesis. Mutations causing PDGFRA constitutive activation have been found in gastrointestinal stromal tumors lacking KIT mutations (PubMed:12522257). .
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. / Contains 5 Ig-like C2-type (immunoglobulin-like) domains. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
N-glycosylated. / Ubiquitinated, leading to its degradation. / Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-731 and Tyr-742 is important for interaction with PIK3R1. Phosphorylation at Tyr-720 and Tyr-754 is important for interaction with PTPN11. Phosphorylation at Tyr-762 is important for interaction with CRK. Phosphorylation at Tyr-572 and Tyr-574 is important for interaction with SRC and SRC family members. Phosphorylation at Tyr-988 and Tyr-1018 is important for interaction with PLCG1.
|Protein cellular localization|| |
Cell membrane / Single-pass type I membrane protein
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St John’s Laboratory Ltd.
|Product type|| |
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