Rabbit Polyclonal Phospho-PGR-S190 antibody (AP0147)

$99.00$259.00

Reactivity: Human
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit Polyclonal Phospho-PGR-S190 antibody (AP0147)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000
IHC 1:50 – 1:100
IF 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-PGR-S190 antibody.

Alternative names:

Check alternative names for the antibody

Expand

PR antibody; NR3C3 antibody;
NR3C3 antibody|Nuclear receptor subfamily 3 group C member 3 antibody|PGR antibody|PR antibody|PRA antibody|PRB antibody|PRGR_HUMAN antibody|Progesterone receptor antibody|Progestin receptor form A antibody|Progestin receptor form B antibody|Anti-Progesterone Receptor antibody [Alpha PR6] – ChIP Grade (ab2765)

SCBT cat No: sc-57553|sc-56862|sc-32629|sc-377544|sc-11768|sc-16795|sc-12891|sc-130604|sc-377573|sc-101787|sc-31714|sc-6118|

Name

Rabbit Polyclonal Phospho-PGR-S190 antibody

Catalogue No.

AP0147

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S190 of human PGR

Host

Rabbit

Applications

WB, IHC, IF

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

99kDa

Formulation

Phospho-PGR-S190 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PR antibody; NR3C3 antibody;

Protein names

PR; NR3C3;

Protein function

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.; Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.; Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Protein sequence and domain

Belongs to the nuclear hormone receptor family. NR3 subfamily.; Contains 1 nuclear receptor DNA-binding domain. Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Protein post-translational modifications

Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.; Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.; Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.; Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

Protein cellular localization

Nucleus. Cytoplasm. Note=Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases.; Isoform A: Nucleus. Cytoplasm. Note=Mainly nuclear.; Isoform 4: Mitochondrion outer membrane .

Background

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Lymphocyte-Signaling/">Lymphocyte Signaling</a>><a href="http://antibodyplus.com/product-tag/PR/">PR</a><br><a href="http://antibodyplus.com/product-tag/Lymphocyte-Signaling/"&gt; (View all antibody categories related to Lymphocyte Signaling)</a>

Note

AntibodyPlus can customize Phospho-PGR-S190 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below: