Rabbit Polyclonal Phospho-PI 3-kinase p85/p55 (Y467/199) antibody (STJ90384)

$99.00$319.00

Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-PI 3-kinase p85/p55 (Y467/199) antibody (STJ90384)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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PIK3R1 antibody, GRB1 antibody,|GRB 1 antibody|GRB1 antibody|p85 alpha antibody|p85 antibody|P85A_HUMAN antibody|Phosphatidylinositol 3 kinase associated p 85 alpha antibody|Phosphatidylinositol 3 kinase regulatory 1 antibody|Phosphatidylinositol 3 kinase regulatory subunit alpha antibody|Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 (p85 alpha) antibody|Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody|Phosphatidylinositol 3-kinase regulatory subunit alpha antibody|Phosphoinositide 3 kinase regulatory subunit 1 (alpha) antibody|Phosphoinositide 3 kinase regulatory subunit 1 (p85 alpha) antibody|Phosphoinositide 3 kinase regulatory subunit 1 antibody|Phosphoinositide 3 kinase regulatory subunit polypeptide 1 (p85 alpha) antibody|PI3 kinase p85 subunit alpha antibody|PI3-kinase regulatory subunit alpha antibody|PI3-kinase subunit p85-alpha antibody|PI3K antibody|PI3K regulatory subunit alpha antibody|Pik3r1 antibody|PtdIns 3 kinase p85 alpha antibody|PtdIns-3-kinase regulatory subunit alpha antibody|PtdIns-3-kinase regulatory subunit p85-alpha antibody|Anti-PI3K p85 antibody [M253] (ab86714)
SCBT cat No: sc-71891|sc-71892|sc-1637|sc-376112|sc-377482|sc-31969|sc-31970|sc-56938|sc-423|sc-374534|sc-292114|

Name

PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody

Catalogue No.

STJ90384

Reactivity

Human, Mouse, Rat, Monkey

Specificity

Phospho-PI 3-kinase p85/p55 (Y467/199) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85/p55 protein only when phosphorylated at Y467/199.

Immunogen

Synthesized phospho-peptide derived from PI 3-kinase p85/p55 (phospho Tyr467/199) at AA range 410-490

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

54/83 kDa

Formulation

PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Phosphatidylinositol 3-kinase regulatory subunit alpha antibody, PI3-kinase regulatory subunit alpha antibody, PI3K regulatory subunit alpha antibody, PtdIns-3-kinase regulatory subunit alpha antibody, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody, PI3-kinase subunit p85-alpha antibody, PtdIns-3-kinase regulatory subunit p85-alpha antibody

Database links

Human UniProt/Swiss-Prot:P27986 ;Human Entrez Gene: 5295;

Protein names

Phosphatidylinositol 3-kinase regulatory subunit alpha , PI3-kinase regulatory subunit alpha , PI3K regulatory subunit alpha , PtdIns-3-kinase regulatory subunit alpha , Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha , PI3-kinase subunit p85-alpha , PtdIns-3-kinase regulatory subunit p85-alpha

Protein function

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling . Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement .

Protein tissue specificity

Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

Involvement in disease

Agammaglobulinemia 7, autosomal recessive (AGM7) [MIM:615214]: A primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. . Note: The disease is caused by mutations affecting the gene represented in this entry.; SHORT syndrome (SHORTS) [MIM:269880]: A rare, multisystem disease characterized by short stature, anomalies of the anterior chamber of the eye, characteristic facial features such as triangular facies, lack of facial fat, and hypoplastic nasal alae with overhanging columella, partial lipodystrophy, hernias, hyperextensibility, and delayed dentition. The clinical phenotype can include insulin resistance, nephrocalcinosis, and hearing deficits. Developmental milestones and cognition are normal. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Immunodeficiency 36 (IMD36) [MIM:616005]: A primary immunodeficiency characterized by impaired B-cell function, hypogammaglobulinemia and recurrent infections. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef. / Belongs to the PI3K p85 subunit family. / Contains 1 Rho-GAP domain. / Contains 2 SH2 domains. / Contains 1 SH3 domain.

Protein post-translational modifications

Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. / Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.

Research area

All research areas>Kinases and Phosphatases>PI 3-kinase
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize PI 3-kinase p85/p55 (phospho Tyr467/199) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human lung tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human uterus cancer tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human appendix tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat heart tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat liver tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spinal cord tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat brain tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse heart tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse testis tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse liver tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse colon tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse brain tissue

1: PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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