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Rabbit Polyclonal Phospho-Pim-1 (Y309) antibody (STJ90491)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:40000;
Recommended protocols: check protocols
Click or hover above images to see image description for Pim-1 (phospho Tyr309) Polyclonal Antibody.
Check alternative names for the antibodyExpand
PIM1 antibody,|Oncogene PIM 1 antibody|Oncogene PIM1 antibody|PIM 1 antibody|pim 1 kinase 44 kDa isoform antibody|Pim 1 kinase antibody|pim 1 oncogene (proviral integration site 1) antibody|Pim 1 oncogene antibody|PIM antibody|PIM1 antibody|pim1 kinase 44 kDa isoform antibody|PIM1_HUMAN antibody|Pim2 antibody|PIM3 antibody|Proto oncogene serine/threonine protein kinase Pim 1 antibody|Proto-oncogene serine/threonine-protein kinase Pim-1 antibody|Proviral integration site 1 antibody|Proviral integration site 2 antibody|Anti-PIM1 antibody [EP2645Y] (ab75776)
SCBT cat No: sc-13513|sc-7856|sc-26375|sc-374116|sc-28777|sc-7857|
Pim-1 (phospho Tyr309) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-Pim-1 (Y309) Polyclonal Antibody detects endogenous levels of Pim-1 protein only when phosphorylated at Y309.
Synthesized phospho-peptide derived from Pim-1 (phospho Tyr309) at AA range 250-330
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:40000;
|Molecular weight|| |
Pim-1 (phospho Tyr309) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Pim-1 (phospho Tyr309) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Serine/threonine-protein kinase pim-1 antibody,
|Protein names|| |
Serine/threonine-protein kinase pim-1 ,
|Protein function|| |
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. / ATP + a protein = ADP + a phosphoprotein. / Mg2+
|Protein tissue specificity|| |
Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Autophosphorylated on both serine/threonine and tyrosine residues. Phosphorylated. Interaction with PPP2CA promotes dephosphorylation. / Ubiquitinated, leading to proteasomal degradation.
|Protein cellular localization|| |
Cytoplasm / Nucleus / Cell membrane
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St John’s Laboratory Ltd.
|Product type|| |
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