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Rabbit Polyclonal Phospho-PKC alpha (Y657) antibody (STJ90499)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for PKC alpha (phospho Tyr657) Polyclonal Antibody.
Check alternative names for the antibodyExpand
PRKCA antibody, PKCA antibody, PRKACA antibody,|KPCA_HUMAN antibody|PKC alpha antibody|PKC beta antibody|PKC delta antibody|PKC epsilon antibody|PKC gamma antibody|PKC zeta antibody|PKC-A antibody|PKC-alpha antibody|PKC2 antibody|PKCA antibody|PKCB antibody|PKCD antibody|PKCE antibody|PKCG antibody|PRKCA antibody|PRKCB antibody|PRKCD antibody|PRKCE antibody|PRKCG antibody|PRKCZ antibody|Protein kinase C alpha antibody|Protein kinase C alpha type antibody|Protein kinase C beta antibody|Protein kinase C delta antibody|Protein kinase C epsilon antibody|Protein kinase C gamma antibody|Protein kinase C zeta antibody|Anti-PKC antibody [MC5] (ab31)
SCBT cat No: sc-393218|sc-17781|sc-366126|sc-7262|sc-17769|sc-17804|
PKC alpha (phospho Tyr657) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-PKC alpha (Y657) Polyclonal Antibody detects endogenous levels of PKC alpha protein only when phosphorylated at Y657.
Synthesized phospho-peptide derived from PKC alpha (phospho Tyr657) at AA range 600-680
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000;
|Molecular weight|| |
PKC alpha (phospho Tyr657) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PKC alpha (phospho Tyr657) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Protein kinase C alpha type antibody, PKC-A antibody, PKC-alpha antibody,
|Protein names|| |
Protein kinase C alpha type , PKC-A , PKC-alpha ,
|Protein function|| |
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. / ATP + a protein = ADP + a phosphoprotein. / Ca2+ / Classical (or conventional) PKCs (PRKCA, PRKCB and PRKCG) are activated by calcium and diacylglycerol (DAG) in the presence of phosphatidylserine. Three specific sites; Thr-497 (activation loop of the kinase domain), Thr-638 (turn motif) and Ser-657 (hydrophobic region), need to be phosphorylated for its full activation.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. / Contains 1 AGC-kinase C-terminal domain. / Contains 1 C2 domain. / Contains 2 phorbol-ester/DAG-type zinc fingers. / Contains 1 protein kinase domain.
|Protein cellular localization|| |
Cytoplasm / Cell membrane / Peripheral membrane protein / Mitochondrion membrane / Peripheral membrane protein / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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