Rabbit polyclonal Phospho-PKCalpha/beta II-T638/641 antibody (AP0598)

$69.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal Phospho-PKCalpha/beta II-T638/641 antibody (AP0598)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-PKCalpha/beta II-T638/641 antibody.

Alternative names:

Check alternative names for the antibody

Expand

AAG6 antibody, PKCA antibody, PRKACA antibody, PKC-alpha antibody
KPCA_HUMAN antibody|PKC alpha antibody|PKC beta antibody|PKC delta antibody|PKC epsilon antibody|PKC gamma antibody|PKC zeta antibody|PKC-A antibody|PKC-alpha antibody|PKC2 antibody|PKCA antibody|PKCB antibody|PKCD antibody|PKCE antibody|PKCG antibody|PRKCA antibody|PRKCB antibody|PRKCD antibody|PRKCE antibody|PRKCG antibody|PRKCZ antibody|Protein kinase C alpha antibody|Protein kinase C alpha type antibody|Protein kinase C beta antibody|Protein kinase C delta antibody|Protein kinase C epsilon antibody|Protein kinase C gamma antibody|Protein kinase C zeta antibody|Anti-PKC antibody [MC5] (ab31)

SCBT cat No: sc-393218|sc-17781|sc-366126|sc-7262|sc-17769|sc-17804|

Name

Rabbit polyclonal Phospho-PKCalpha/beta II-T638/641 antibody

Catalogue No.

AP0598

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding T638/641 of human PKCalpha/beta II

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 77kDa/Observed: Refer to figures

Formulation

Phospho-PKCalpha/beta II-T638/641 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-PKCalpha/beta II-T638/641 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

AAG6 antibody, PKCA antibody, PRKACA antibody, PKC-alpha antibody

Database links

Human UniProt/Swiss-Prot:P17252

Protein names

AAG6, PKCA, PRKACA, PKC-alpha

Protein function

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

Protein sequence and domain

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.; Contains 1 AGC-kinase C-terminal domain.; Contains 1 C2 domain.; Contains 2 phorbol-ester/DAG-type zinc fingers.; Contains 1 protein kinase domain.

Protein cellular localization

Cytoplasm . Cell membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein . Nucleus .

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Research area

All research areas>Kinases and Phosphatases>PKC
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Phospho-PKCalpha/beta II-T638/641 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!