Rabbit polyclonal Phospho-PKCdelta/theta-S643/676 antibody (AP0567)

$69.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-PKCdelta/theta-S643/676 antibody (AP0567)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-PKCdelta/theta-S643/676 antibody.

Alternative names:

Check alternative names for the antibody

Expand

MAY1 antibody, PKCD antibody, ALPS3 antibody, CVID9 antibody, nPKC-delta antibody
KPCT_HUMAN antibody|MGC126514 antibody|MGC141919 antibody|nPKC theta antibody|nPKC-theta antibody|nPKCtheta antibody|OTTHUMP00000043364 antibody|OTTHUMP00000043365 antibody|PKC 0 antibody|PKC0 antibody|Prkcq antibody|PRKCT antibody|Protein kinase C theta antibody|Protein kinase C theta type antibody|Protein Kinase Ctheta antibody|Phospho anti-PKC theta (T538) antibody [F4H4L1] (ab203565)

SCBT cat No: sc-81534|sc-212|sc-1875|sc-1680|sc-393218|sc-17781|sc-366126|sc-7262|sc-17769|sc-17804|sc-10800|sc-80|sc-100415|

Name

Rabbit polyclonal Phospho-PKCdelta/theta-S643/676 antibody

Catalogue No.

AP0567

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S643/676 of human PKCdelta/theta

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 78kDa/Observed: Refer to figures

Formulation

Phospho-PKCdelta/theta-S643/676 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-PKCdelta/theta-S643/676 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MAY1 antibody, PKCD antibody, ALPS3 antibody, CVID9 antibody, nPKC-delta antibody

Database links

Human UniProt/Swiss-Prot:Q04759

Protein names

MAY1, PKCD, ALPS3, CVID9, nPKC-delta

Protein function

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates to the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in ‘outside-in’ signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at ‘Ser-504’ and ‘Ser-532’ and negatively regulates its ability to phosphorylate PKB/AKT1.

Protein tissue specificity

Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.

Protein sequence and domain

The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.; Contains 1 AGC-kinase C-terminal domain.; Contains 1 C2 domain.; Contains 2 phorbol-ester/DAG-type zinc fingers.; Contains 1 protein kinase domain.

Protein post-translational modifications

Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PRKCQ upon antigen receptor ligation. Following TCR stimulation, phosphorylated at Tyr-90 and Ser-685.

Protein cellular localization

Cytoplasm. Cell membrane; Peripheral membrane protein. Note: In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.

Research area

All research areas>Kinases and Phosphatases>PKC
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Phospho-PKCdelta/theta-S643/676 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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