Rabbit Polyclonal Phospho-PKD1/2/3 (S738/S742) antibody (STJ90866)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-PKD1/2/3 (S738/S742) antibody (STJ90866)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for PKD1/2/3 (phospho Ser738/S742) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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PRKD1 antibody, PKD antibody, PKD1 antibody, PRKCM antibody,|KPCD1_HUMAN antibody|nPKC D1 antibody|nPKC mu antibody|nPKC-D1 antibody|nPKC-mu antibody|nPKCD1 antibody|nPKCmu antibody|PKC antibody|PKC MU antibody|PKCM antibody|PKCmu antibody|PKD 1 antibody|PKD antibody|PKD1 antibody|PRKCM antibody|PRKD 1 antibody|Prkd1 antibody|Protein kinase C mu antibody|Protein kinase C mu type antibody|Protein kinase D antibody|Protein kinase D1 antibody|Serine/threonine protein kinase D1 antibody|Serine/threonine-protein kinase D1 antibody|Phospho anti-PKC mu (Y463) antibody (ab59415)
SCBT cat No: sc-639|sc-935|sc-292444|sc-376024|

 

Name

PKD1/2/3 (phospho Ser738/S742) Polyclonal Antibody

Catalogue No.

STJ90866

Reactivity

Human, Mouse, Rat

Specificity

Phospho-PKD1/2/3 (S738/S742) Polyclonal Antibody detects endogenous levels of PKD1/2/3 protein only when phosphorylated at S738/S742.

Immunogen

Synthesized phospho-peptide derived from PKD1/2/3 (phospho Ser738/S742) at AA range 680-760

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

101 kDa

Formulation

PKD1/2/3 (phospho Ser738/S742) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

PKD1/2/3 (phospho Ser738/S742) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Serine/threonine-protein kinase D1 antibody, Protein kinase C mu type antibody, Protein kinase D antibody, nPKC-D1 antibody, nPKC-mu antibody

Database links

Human UniProt/Swiss-Prot:Q15139 ;Human Entrez Gene: 5587;

Protein names

Serine/threonine-protein kinase D1 , Protein kinase C mu type , Protein kinase D , nPKC-D1 , nPKC-mu

Protein function

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and trafficking, cell survival through NF-kappa-B activation, cell migration, cell differentiation by mediating HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) signaling, and plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis and flagellin-stimulated inflammatory response. Phosphorylates the epidermal growth factor receptor (EGFR) on dual threonine residues, which leads to the suppression of epidermal growth factor (EGF)-induced MAPK8/JNK1 activation and subsequent JUN phosphorylation. Phosphorylates RIN1, inducing RIN1 binding to 14-3-3 proteins YWHAB, YWHAE and YWHAZ and increased competition with RAF1 for binding to GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). Acts downstream of the heterotrimeric G-protein beta/gamma-subunit complex to maintain the structural integrity of the Golgi membranes, and is required for protein transport along the secretory pathway. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane. May act by activating the lipid kinase phosphatidylinositol 4-kinase beta (PI4KB) at the TGN for the local synthesis of phosphorylated inositol lipids, which induces a sequential production of DAG, phosphatidic acid (PA) and lyso-PA (LPA) that are necessary for membrane fission and generation of specific transport carriers to the cell surface. Under oxidative stress, is phosphorylated at Tyr-463 via SRC-ABL1 and contributes to cell survival by activating IKK complex and subsequent nuclear translocation and activation of NFKB1. Involved in cell migration by regulating integrin alpha-5/beta-3 recycling and promoting its recruitment in newly forming focal adhesion. In osteoblast differentiation, mediates the bone morphogenic protein 2 (BMP2)-induced nuclear export of HDAC7, which results in the inhibition of HDAC7 transcriptional repression of RUNX2. In neurons, plays an important role in neuronal polarity by regulating the biogenesis of TGN-derived dendritic vesicles, and is involved in the maintenance of dendritic arborization and Golgi structure in hippocampal cells. May potentiate mitogenesis induced by the neuropeptide bombesin or vasopressin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. Plays an important role in the proliferative response induced by low calcium in keratinocytes, through sustained activation of MAPK1/3 (ERK1/2) pathway. Downstream of novel PKC signaling, plays a role in cardiac hypertrophy by phosphorylating HDAC5, which in turn triggers XPO1/CRM1-dependent nuclear export of HDAC5, MEF2A transcriptional activation and induction of downstream target genes that promote myocyte hypertrophy and pathological cardiac remodeling. Mediates cardiac troponin I (TNNI3) phosphorylation at the PKA sites, which results in reduced myofilament calcium sensitivity, and accelerated crossbridge cycling kinetics. The PRKD1-HDAC5 pathway is also involved in angiogenesis by mediating VEGFA-induced specific subset of gene expression, cell migration, and tube formation. In response to VEGFA, is necessary and required for HDAC7 phosphorylation which induces HDAC7 nuclear export and endothelial cell proliferation and migration. During apoptosis induced by cytarabine and other genotoxic agents, PRKD1 is cleaved by caspase-3 at Asp-378, resulting in activation of its kinase function and increased sensitivity of cells to the cytotoxic effects of genotoxic agents. In epithelial cells, is required for transducing flagellin-stimulated inflammatory responses by binding and phosphorylating TLR5, which contributes to MAPK14/p38 activation and production of inflammatory cytokines. May play a role in inflammatory response by mediating activation of NF-kappa-B. May be involved in pain transmission by directly modulating TRPV1 receptor. Plays a role in activated KRAS-mediated stabilization of ZNF304 in colorectal cancer (CRC) cells . / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domain 1 binds DAG with high affinity and appears to play the dominant role in mediating translocation to the cell membrane and trans-Golgi network. Phorbol-ester/DAG-type domain 2 binds phorbol ester with higher affinity. Autophosphorylation of Ser-742 and phosphorylation of Ser-738 by PKC relieves auto-inhibition by the PH domain. Phosphorylation on Tyr-463 by the SRC-ABL1 pathway in response to oxidative stress, is also required for activation. Activated by DAPK1 under oxidative stress.

Protein tissue specificity

Up-regulated by the intestine-specific transcription factor CDX1 in a activated KRAS-dependent manner in colorectal cancer (CRC) cells (PubMed:24623306).

Protein sequence and domain

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. / Contains 1 PH domain. / Contains 2 phorbol-ester/DAG-type zinc fingers. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylated at Ser-397 and Ser-401 by MAPK13 during regulation of insulin secretion in pancreatic beta cells. Phosphorylated by DAPK1. Phosphorylated by ABL at Tyr-463, which primes the kinase in response to oxidative stress, and promotes a second step activating phosphorylation at Ser-738/Ser-742 by PKRD.

Protein cellular localization

Cytoplasm / Cell membrane / Golgi apparatus > trans-Golgi network

Research area

All research areas>Kinases and Phosphatases>PKD
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize PKD1/2/3 (phospho Ser738/S742) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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