Rabbit Polyclonal Phospho-PKD2 (S876) antibody (STJ90501)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-PKD2 (S876) antibody (STJ90501)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for PKD2 (phospho Ser876) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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PRKD2 antibody, PKD2 antibody, HSPC187 antibody,|DKFZp586E0820 antibody|HSPC187 antibody|KPCD2_HUMAN antibody|nPKC D2 antibody|nPKC-D2 antibody|PKD2 antibody|PRKD 2 antibody|Prkd2 antibody|Protein kinase D2 antibody|Serine/threonine protein kinase D2 antibody|Serine/threonine-protein kinase D2 antibody|Anti-Protein Kinase D2 antibody (ab7281)
SCBT cat No: sc-100415|sc-74838|sc-74839|sc-374344|sc-374213|

 

Name

PKD2 (phospho Ser876) Polyclonal Antibody

Catalogue No.

STJ90501

Reactivity

Human, Mouse, Rat

Specificity

Phospho-PKD2 (S876) Polyclonal Antibody detects endogenous levels of PKD2 protein only when phosphorylated at S876.

Immunogen

Synthesized phospho-peptide derived from PKD2 (phospho Ser876) at AA range 810-890

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

96 kDa

Formulation

PKD2 (phospho Ser876) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

PKD2 (phospho Ser876) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Serine/threonine-protein kinase D2 antibody, nPKC-D2 antibody

Database links

Human UniProt/Swiss-Prot:Q9BZL6;Mouse UniPort/Swiss-Prot: Q8BZ03;Rat UniProt/Swiss-Port: Q5XIS9;Human Entrez Gene: 25865;Mouse Entrez Gene: 101540;Rat Entrez Gene: Rn.15035

Protein names

Serine/threonine-protein kinase D2 , nPKC-D2

Protein function

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells. Plays a regulatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resulting in vascular endothelial growth factor A (VEGFA) secretion. / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain.

Protein tissue specificity

Widely expressed.

Protein sequence and domain

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. / Contains 1 PH domain. / Contains 2 phorbol-ester/DAG-type zinc fingers. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylation of Ser-876 correlates with the activation status of the kinase. Ser-706 is probably phosphorylated by PKC. Phosphorylation at Ser-244 by CSNK1D and CSNK1E promotes nuclear localization and substrate targeting. Phosphorylation at Ser-244, Ser-706 and Ser-710 is required for nuclear localization. Phosphorylated at Tyr-438 by ABL1 in response to oxidative stress.

Protein cellular localization

Cytoplasm / Cell membrane / Nucleus / Golgi apparatus > trans-Golgi network

Research area

All research areas>Kinases and Phosphatases>PKD
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize PKD2 (phospho Ser876) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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