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Rabbit Polyclonal Phospho-PKR (T258) antibody (STJ90956)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;
Recommended protocols: check protocols
Click or hover above images to see image description for PKR (phospho Thr258) Polyclonal Antibody.
Check alternative names for the antibodyExpand
EIF2AK2 antibody, PKR antibody, PRKR antibody,|Double stranded RNA activated protein kinase; antibody|E2AK2_HUMAN antibody|eIF-2A protein kinase 2 antibody|EIF2AK1 antibody|EIF2AK2 antibody|Eukaryotic translation initiation factor 2 alpha kinase 2 antibody|Eukaryotic translation initiation factor 2-alpha kinase 2 antibody|HGNC:9437 antibody|Interferon induced double stranded RNA activated protein kinase antibody|Interferon inducible elF2 alpha kinase antibody|Interferon inducible RNA dependent protein kinase antibody|Interferon-induced, double-stranded RNA-activated protein kinase antibody|Interferon-inducible RNA-dependent protein kinase antibody|MGC126524 antibody|P1/eIF-2A protein kinase antibody|P1/eIF2A protein kinase antibody|p68 kinase antibody|PKR antibody|PPP1R83 antibody|PRKR antibody|Protein kinase interferon inducible double stranded RNA dependent antibody|Protein kinase RNA activated antibody|Protein kinase RNA-activated antibody|Protein phosphatase 1 regulatory subunit 83 antibody|Serine/threonine protein kinase TIK antibody|Tyrosine protein kinase EIF2AK2 antibody|Anti-PKR antibody [Y117] (ab32506) KO Validated
SCBT cat No: sc-136038|sc-136352|sc-393038|sc-6282|sc-708|sc-100378|sc-514626|sc-366778|sc-707|sc-366777|sc-1702|sc-709|sc-54313|sc-365696|
PKR (phospho Thr258) Polyclonal Antibody
|Catalogue No.|| |
Phospho-PKR (T258) Polyclonal Antibody detects endogenous levels of PKR protein only when phosphorylated at T258.
Synthesized phospho-peptide derived from PKR (phospho Thr258) at AA range 200-280
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;
|Molecular weight|| |
PKR (phospho Thr258) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PKR (phospho Thr258) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Interferon-induced, double-stranded RNA-activated protein kinase antibody, Eukaryotic translation initiation factor 2-alpha kinase 2 antibody, eIF-2A protein kinase 2 antibody, Interferon-inducible RNA-dependent protein kinase antibody, P1/eIF-2A protein kinase antibody, Protein kinase RNA-activated antibody, PKR antibody, Protein kinase R antibody, Tyrosine-protein kinase EIF2AK2 antibody, p68 kinase antibody
|Protein names|| |
Interferon-induced, double-stranded RNA-activated protein kinase , Eukaryotic translation initiation factor 2-alpha kinase 2 , eIF-2A protein kinase 2 , Interferon-inducible RNA-dependent protein kinase , P1/eIF-2A protein kinase , Protein kinase RNA-activated , PKR , Protein kinase R , Tyrosine-protein kinase EIF2AK2 , p68 kinase
|Protein function|| |
IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at ‘Tyr-4’ upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at ‘Ser-312’ and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin. / ATP + a protein = ADP + a phosphoprotein. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Initially produced in an inactive form and is activated by binding to viral dsRNA, which causes dimerization and autophosphorylation in the activation loop and stimulation of function. ISGylation can activate it in the absence of viral infection. Can also be activated by heparin, proinflammatory stimuli, growth factors, cytokines, oxidative stress and the cellular protein PRKRA. Activity is markedly stimulated by manganese ions. Activation is blocked by the viral components HIV-1 Tat protein and large amounts of HIV-1 trans-activation response (TAR) RNA element as well as by the cellular proteins TARBP2, DUS2L, NPM1, NCK1 and ADAR. Down-regulated by Toscana virus (TOS) and Rift valley fever virus (RVFV) NSS which promote its proteasomal degradation. Inhibited by vaccinia virus protein E3, probably via dsRNA sequestering.
|Protein tissue specificity|| |
Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcinomas,and which correlates with tumor progression and invasiveness or risk of progression.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. / Contains 2 DRBM (double-stranded RNA-binding) domains. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Autophosphorylated on several Ser, Thr and Tyr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. Tyrosine autophosphorylation is essential for efficient dsRNA-binding, dimerization, and kinase activation.
|Protein cellular localization|| |
Cytoplasm / Nucleus / Cytoplasm > perinuclear region
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St John’s Laboratory Ltd.
|Product type|| |
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