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Rabbit Polyclonal Phospho-PR (S294) antibody (STJ90691)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for PR (phospho Ser294) Polyclonal Antibody.
Check alternative names for the antibodyExpand
PGR antibody, NR3C3 antibody,|NR3C3 antibody|Nuclear receptor subfamily 3 group C member 3 antibody|PGR antibody|PR antibody|PRA antibody|PRB antibody|PRGR_HUMAN antibody|Progesterone receptor antibody|Progestin receptor form A antibody|Progestin receptor form B antibody|Anti-Progesterone Receptor antibody [Alpha PR6] – ChIP Grade (ab2765)
SCBT cat No: sc-57553|sc-56862|sc-32629|sc-377544|sc-11768|sc-16795|sc-12891|sc-130604|sc-377573|sc-101787|sc-31714|sc-6118|
PR (phospho Ser294) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-PR (S294) Polyclonal Antibody detects endogenous levels of PR protein only when phosphorylated at S294.
Synthesized phospho-peptide derived from PR (phospho Ser294) at AA range 230-310
WB, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000;
|Molecular weight|| |
PR (phospho Ser294) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PR (phospho Ser294) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Progesterone receptor antibody, PR antibody, Nuclear receptor subfamily 3 group C member 3 antibody
|Protein names|| |
Progesterone receptor , PR , Nuclear receptor subfamily 3 group C member 3
|Protein function|| |
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. / Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation. / Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
|Protein sequence and domain|| |
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. / Belongs to the nuclear hormone receptor family. NR3 subfamily. / Contains 1 nuclear receptor DNA-binding domain.
|Protein post-translational modifications|| |
Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G2/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1. / Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294. / Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294. / Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
|Protein cellular localization|| |
Nucleus / Cytoplasm / Nucleus / Cytoplasm / Mitochondrion outer membrane
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St John’s Laboratory Ltd.
|Product type|| |
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