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Rabbit Polyclonal Phospho-PRAS40 (T246) antibody (STJ90462)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for PRAS40 (phospho Thr246) Polyclonal Antibody.
Check alternative names for the antibodyExpand
AKT1S1 antibody, PRAS40 antibody,|40 kDa proline rich AKT substrate antibody|40 kDa proline-rich AKT substrate antibody|AKT1 S1 antibody|AKT1 substrate 1 (proline rich) antibody|AKT1 substrate 1 antibody|AKT1S 1 antibody|AKT1S1 antibody|AKTS1_HUMAN antibody|Lobe antibody|MGC2865 antibody|PRAS 40 antibody|PRAS antibody|PRAS40 antibody|Proline rich akt substrate antibody|Proline rich Akt substrate 40 kDa antibody|Proline-rich AKT1 substrate 1 antibody|Phospho anti-PRAS40 (T246) antibody (ab214100)
SCBT cat No: sc-32629|sc-377544|
PRAS40 (phospho Thr246) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-PRAS40 (T246) Polyclonal Antibody detects endogenous levels of PRAS40 protein only when phosphorylated at T246.
Synthesized phospho-peptide derived from PRAS40 (phospho Thr246) at AA range 180-260
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000;
|Molecular weight|| |
PRAS40 (phospho Thr246) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PRAS40 (phospho Thr246) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Proline-rich AKT1 substrate 1 antibody, 40 kDa proline-rich AKT substrate antibody
|Protein names|| |
Proline-rich AKT1 substrate 1 , 40 kDa proline-rich AKT substrate
|Protein function|| |
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at ‘Thr-389’, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
|Protein tissue specificity|| |
Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
|Protein post-translational modifications|| |
Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
|Protein cellular localization|| |
Cytoplasm > cytosol
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St John’s Laboratory Ltd.
|Product type|| |
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