Rabbit Polyclonal Phospho-PRKDC-T2609 antibody (AP0434)

$99.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-PRKDC-T2609 antibody (AP0434)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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HYRC antibody; p350 antibody; DNAPK antibody; DNPK1 antibody; HYRC1 antibody; XRCC7 antibody; DNA-PKcs antibody;
DNA dependent protein kinase catalytic subunit antibody|DNA PK catalytic subunit antibody|DNA-dependent protein kinase catalytic subunit antibody|DNA-PK catalytic subunit antibody|DNA-PKcs antibody|DNAPK antibody|DNAPK catalytic subunit antibody|DNPK 1 antibody|DNPK1 antibody|Hyper radiosensitivity of murine scid mutation, complementing 1 antibody|HYRC 1 antibody|HYRC antibody|HYRC1 antibody|IMD26 antibody|p350 antibody|p460 antibody|PKRDC antibody|PRKDC antibody|PRKDC_HUMAN antibody|Protein Kinase DNA Activated Catalytic Polypeptide antibody|XRCC 7 antibody|XRCC7 antibody|Anti-DNA PKcs antibody [18-2] (ab1832)

SCBT cat No: sc-390698|sc-390849|sc-5282|sc-376207|

Name

Rabbit Polyclonal Phospho-PRKDC-T2609 antibody

Catalogue No.

AP0434

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding T2609 of human PRKDC

Host

Rabbit

Applications

WB

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

450kDa

Formulation

Phospho-PRKDC-T2609 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

HYRC antibody; p350 antibody; DNAPK antibody; DNPK1 antibody; HYRC1 antibody; XRCC7 antibody; DNA-PKcs antibody;

Protein names

HYRC; p350; DNAPK; DNPK1; HYRC1; XRCC7; DNA-PKcs;

Protein function

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.

Protein sequence and domain

Belongs to the PI3/PI4-kinase family.; Contains 1 FAT domain.; Contains 1 FATC domain.; Contains 2 HEAT repeats.; Contains 1 PI3K/PI4K domain.; Contains 3 TPR repeats.

Protein post-translational modifications

Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647. Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.; S-nitrosylated by GAPDH.; Polyubiquitinated by RNF144A, leading to proteasomal degradation.

Protein cellular localization

Nucleus . Nucleus, nucleolus .

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Tumor-SuppressorApoptosis/">Tumor Suppressor/Apoptosis</a>><a href="http://antibodyplus.com/product-tag/DNA-PK/">DNA-PK</a><br><a href="http://antibodyplus.com/product-tag/Tumor-SuppressorApoptosis/"&gt; (View all antibody categories related to Tumor Suppressor/Apoptosis)</a>

Note

AntibodyPlus can customize Phospho-PRKDC-T2609 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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