Rabbit Polyclonal Phospho-PTEN-S380 antibody (AP0436)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-PTEN-S380 antibody (AP0436)

Supplier: ABclonal Inc.

Recommended applications: IHC,IF

Recommended dilution:

IHC 1:50 – 1:200
IF 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-PTEN-S380 antibody.

Alternative names:

Check alternative names for the antibody

Expand

BZS antibody; DEC antibody; CWS1 antibody; GLM2 antibody; MHAM antibody; TEP1 antibody; MMAC1 antibody; PTEN1 antibody; 10q23del antibody;
10q23del antibody|BZS antibody|DEC antibody|GLM2 antibody|MGC11227 antibody|MHAM antibody|MMAC1 antibody|MMAC1 phosphatase and tensin homolog deleted on chromosome 10 antibody|Mutated in multiple advanced cancers 1 antibody|Phosphatase and tensin homolog antibody|Phosphatase and tensin like protein antibody|Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN antibody|Pten antibody|PTEN_HUMAN antibody|PTEN1 antibody|TEP1 antibody|Anti-PTEN antibody [Y184] (ab32199)

SCBT cat No: sc-377573|sc-101787|sc-31714|sc-6118|sc-374106|sc-293142|sc-81512|sc-101790|sc-11767|sc-16822|sc-16824|sc-16823|sc-16825|sc-374260|

Name

Rabbit Polyclonal Phospho-PTEN-S380 antibody

Catalogue No.

AP0436

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding S380 of human PTEN

Host

Rabbit

Applications

IHC, IF

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

47kDa

Formulation

Phospho-PTEN-S380 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

BZS antibody; DEC antibody; CWS1 antibody; GLM2 antibody; MHAM antibody; TEP1 antibody; MMAC1 antibody; PTEN1 antibody; 10q23del antibody;

Protein names

BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del;

Protein function

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4 (PubMed:26504226). The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement.; Isoform alpha: Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.

Protein tissue specificity

Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Protein sequence and domain

Contains 1 C2 tensin-type domain.; Contains 1 phosphatase tensin-type domain. The C2 domain binds phospholipid membranes in vitro in a Ca(2+)-independent manner; this binding is important for its tumor suppressor function.

Protein post-translational modifications

Constitutively phosphorylated by CK2 under normal conditions. Phosphorylated in vitro by MAST1, MAST2, MAST3 and STK11. Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit or promote PDZ-binding. Phosphorylation at Tyr-336 by FRK/PTK5 protects this protein from ubiquitin-mediated degradation probably by inhibiting its binding to NEDD4. Phosphorylation by ROCK1 is essential for its stability and activity. Phosphorylation by PLK3 promotes its stability and prevents its degradation by the proteasome.; Monoubiquitinated; monoubiquitination is increased in presence of retinoic acid. Deubiquitinated by USP7; leading to its nuclear exclusion. Monoubiquitination of one of either Lys-13 and Lys-289 amino acid is sufficient to modulate PTEN compartmentalization. Ubiquitinated by XIAP/BIRC4.

Protein cellular localization

Cytoplasm. Nucleus. Nucleus, PML body. Note=Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies. XIAP/BIRC4 promotes its nuclear localization.; Isoform alpha: Secreted . Note=May be secreted via a classical signal peptide and reenter into cells with the help of a poly-Arg motif.

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Tumor-SuppressorApoptosis/">Tumor Suppressor/Apoptosis</a>><a href="http://antibodyplus.com/product-tag/PTEN/">PTEN</a><br><a href="http://antibodyplus.com/product-tag/Tumor-SuppressorApoptosis/"&gt; (View all antibody categories related to Tumor Suppressor/Apoptosis)</a>

Note

AntibodyPlus can customize Phospho-PTEN-S380 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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