Rabbit Polyclonal Phospho-RARalpha (S77) antibody (STJ90963)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-RARalpha (S77) antibody (STJ90963)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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RARA antibody, NR1B1 antibody,|NR1B1 antibody|Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein antibody|Nuclear receptor subfamily 1 group B member 1 antibody|Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form antibody|RAR alpha antibody|RAR antibody|RAR-alpha antibody|rara antibody|RARA_HUMAN antibody|RARalpha antibody|RARalpha1 antibody|Retinoic acid nuclear receptor alpha variant 1 antibody|Retinoic acid nuclear receptor alpha variant 2 antibody|Retinoic acid receptor alpha antibody|Retinoic acid receptor alpha polypeptide antibody|Anti-Retinoic Acid Receptor alpha antibody (ab28767)
SCBT cat No: sc-293417|sc-551|sc-366090|sc-15040|

 

Name

RARalpha (phospho Ser77) Polyclonal Antibody

Catalogue No.

STJ90963

Reactivity

Human, Mouse, Rat

Specificity

Phospho-RARalpha (S77) Polyclonal Antibody detects endogenous levels of RARalpha protein only when phosphorylated at S77.

Immunogen

Synthesized phospho-peptide derived from RARalpha (phospho Ser77) at AA range 20-100

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

45 kDa

Formulation

RARalpha (phospho Ser77) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

RARalpha (phospho Ser77) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Retinoic acid receptor alpha antibody, RAR-alpha antibody, Nuclear receptor subfamily 1 group B member 1 antibody

Database links

Human UniProt/Swiss-Prot:P10276;Mouse UniPort/Swiss-Prot: P11416;Rat UniProt/Swiss-Port: Q499N1;Human Entrez Gene: 5914;Mouse Entrez Gene: 19401;Rat Entrez Gene: Rn.91057

Protein names

Retinoic acid receptor alpha , RAR-alpha , Nuclear receptor subfamily 1 group B member 1

Protein function

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5′-AGGTCA-3′ sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.

Involvement in disease

Note: Chromosomal aberrations involving RARA are commonly found in acute promyelocytic leukemia. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. The PML-RARA oncoprotein requires both the PML ring structure and coiled-coil domain for both interaction with UBE2I, nuclear microspeckle location and sumoylation. In addition, the coiled-coil domain functions in blocking RA-mediated transactivation and cell differentiation. .

Protein sequence and domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. / Belongs to the nuclear hormone receptor family. NR1 subfamily. / Contains 1 nuclear receptor DNA-binding domain.

Protein post-translational modifications

Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosphorylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling. / Sumoylated with SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity. / Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation. / Ubiquitinated.

Protein cellular localization

Nucleus / Cytoplasm

Research area

All research areas>Steroid Receptors>RAR alpha
(View all antibody categories related to Steroid Receptors)

Note

AntibodyPlus can customize RARalpha (phospho Ser77) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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