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Rabbit Polyclonal Phospho-RARalpha (S77) antibody (STJ90963)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
RARA antibody, NR1B1 antibody,|NR1B1 antibody|Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein antibody|Nuclear receptor subfamily 1 group B member 1 antibody|Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form antibody|RAR alpha antibody|RAR antibody|RAR-alpha antibody|rara antibody|RARA_HUMAN antibody|RARalpha antibody|RARalpha1 antibody|Retinoic acid nuclear receptor alpha variant 1 antibody|Retinoic acid nuclear receptor alpha variant 2 antibody|Retinoic acid receptor alpha antibody|Retinoic acid receptor alpha polypeptide antibody|Anti-Retinoic Acid Receptor alpha antibody (ab28767)
SCBT cat No: sc-293417|sc-551|sc-366090|sc-15040|
RARalpha (phospho Ser77) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-RARalpha (S77) Polyclonal Antibody detects endogenous levels of RARalpha protein only when phosphorylated at S77.
Synthesized phospho-peptide derived from RARalpha (phospho Ser77) at AA range 20-100
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
|Molecular weight|| |
RARalpha (phospho Ser77) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
RARalpha (phospho Ser77) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Retinoic acid receptor alpha antibody, RAR-alpha antibody, Nuclear receptor subfamily 1 group B member 1 antibody
|Protein names|| |
Retinoic acid receptor alpha , RAR-alpha , Nuclear receptor subfamily 1 group B member 1
|Protein function|| |
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5′-AGGTCA-3′ sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.
|Involvement in disease|| |
Note: Chromosomal aberrations involving RARA are commonly found in acute promyelocytic leukemia. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. The PML-RARA oncoprotein requires both the PML ring structure and coiled-coil domain for both interaction with UBE2I, nuclear microspeckle location and sumoylation. In addition, the coiled-coil domain functions in blocking RA-mediated transactivation and cell differentiation. .
|Protein sequence and domain|| |
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. / Belongs to the nuclear hormone receptor family. NR1 subfamily. / Contains 1 nuclear receptor DNA-binding domain.
|Protein post-translational modifications|| |
Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosphorylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling. / Sumoylated with SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity. / Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation. / Ubiquitinated.
|Protein cellular localization|| |
Nucleus / Cytoplasm
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St John’s Laboratory Ltd.
|Product type|| |
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