Rabbit polyclonal Phospho-Rb-S811 antibody (AP0089)

$69.00$259.00

Reactivity: Human
Applications: WB,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-Rb-S811 antibody (AP0089)

Supplier: ABclonal Inc.

Recommended applications: WB,IF

Recommended dilution:

WB 1:1000 – 1:2000 IF 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-Rb-S811 antibody.

Alternative names:

Check alternative names for the antibody

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RB1 antibody, RB antibody, pRb antibody, OSRC antibody, pp110 antibody, p105-Rb antibody
OSRC antibody|Osteosarcoma antibody|p105-Rb antibody|P105RB antibody|PP105 antibody|pp110 antibody|PPP1R130 antibody|pRb antibody|Prepro retinoblastoma associated protein antibody|Protein phosphatase 1 regulatory subunit 130 antibody|Rb antibody|RB_HUMAN antibody|RB1 antibody|RB1 gene antibody|Retinoblastoma 1 antibody|Retinoblastoma suspectibility protein antibody|Retinoblastoma-associated protein antibody|Phospho anti-Rb (S780) antibody (ab47763)

SCBT cat No: sc-74562|

Name

Rabbit polyclonal Phospho-Rb-S811 antibody

Catalogue No.

AP0089

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S811 of human RB

Host

Rabbit

Applications

WB, IF

Recommended dilution

WB 1:1000 – 1:2000
IF 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 102kDa/Observed: Refer to Figures

Formulation

Phospho-Rb-S811 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-Rb-S811 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

RB1 antibody, RB antibody, pRb antibody, OSRC antibody, pp110 antibody, p105-Rb antibody

Database links

Human UniProt/Swiss-Prot:P06400

Protein names

RB1, RB, pRb, OSRC, pp110, p105-Rb

Protein function

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 ‘Lys-20’ trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1’s activity.

Protein tissue specificity

Expressed in the retina.

Protein sequence and domain

The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.Belongs to the retinoblastoma protein (RB) family.

Protein post-translational modifications

Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis (By similarity).; N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.; Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.

Protein cellular localization

Nucleus .

Background

The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

Note

AntibodyPlus can customize Phospho-Rb-S811 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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