Rabbit Polyclonal Phospho-RB1-S780 antibody (AP0444)

$99.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-RB1-S780 antibody (AP0444)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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RB antibody; pRb antibody; OSRC antibody; pp110 antibody; p105-Rb antibody;
OSRC antibody|Osteosarcoma antibody|p105-Rb antibody|P105RB antibody|PP105 antibody|pp110 antibody|PPP1R130 antibody|pRb antibody|Prepro retinoblastoma associated protein antibody|Protein phosphatase 1 regulatory subunit 130 antibody|Rb antibody|RB_HUMAN antibody|RB1 antibody|RB1 gene antibody|Retinoblastoma 1 antibody|Retinoblastoma suspectibility protein antibody|Retinoblastoma-associated protein antibody|Phospho anti-Rb (S780) antibody (ab47763)

SCBT cat No: sc-74562|

Name

Rabbit Polyclonal Phospho-RB1-S780 antibody

Catalogue No.

AP0444

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S780 of human RB1

Host

Rabbit

Applications

WB

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

110kDa

Formulation

Phospho-RB1-S780 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

RB antibody; pRb antibody; OSRC antibody; pp110 antibody; p105-Rb antibody;

Protein names

RB; pRb; OSRC; pp110; p105-Rb;

Protein function

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Protein tissue specificity

Expressed in the retina.

Protein sequence and domain

Belongs to the retinoblastoma protein (RB) family. The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.

Protein post-translational modifications

Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis (By similarity).; N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.; Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.

Protein cellular localization

Nucleus .

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Tumor-SuppressorApoptosis/">Tumor Suppressor/Apoptosis</a>><a href="http://antibodyplus.com/product-tag/p53/">p53</a><br><a href="http://antibodyplus.com/product-tag/Tumor-SuppressorApoptosis/"&gt; (View all antibody categories related to Tumor Suppressor/Apoptosis)</a>

Note

AntibodyPlus can customize Phospho-RB1-S780 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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