Rabbit Polyclonal Phospho-RPA32 (T21) antibody (STJ90903)


Reactivity: Human, Mouse
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-RPA32 (T21) antibody (STJ90903)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for RPA32 (phospho Thr21) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


RPA2 antibody, REPA2 antibody, RPA32 antibody, RPA34 antibody,|60S acidic ribosomal protein P1 antibody|AA409079 antibody|AI325195 antibody|AU020965 antibody|ik:tdsubc_2g1 antibody|M(2)21C antibody|MGC137236 antibody|OTTHUMP00000004008 antibody|p32 antibody|p34 antibody|RCJMB04_6d17 replication protein A2, 32kDa antibody|REPA2 antibody|Replication factor A protein 2 antibody|Replication protein A 32 kDa subunit antibody|Replication protein A 32kDa subunit antibody|Replication protein A 34 kDa subunit antibody|Replication protein A antibody|Replication Protein A2 (32kDa) antibody|Replication protein A2 antibody|Replication protein A2, 32kDa antibody|RF-A protein 2 antibody|Rf-A2 antibody|RFA antibody|RFA2_HUMAN antibody|RP-A p32 antibody|RP-A p34 antibody|RP21C antibody|RPA 2 antibody|RPA 32 antibody|RPA antibody|Rpa2 antibody|RPA32 antibody|RPA34 antibody|RpLP1 antibody|RpP2 antibody|xx:tdsubc_2g1 antibody|zgc:109822 antibody|Anti-RPA32/RPA2 antibody [9H8] (ab2175)
SCBT cat No: sc-56770|sc-271578|sc-14692|sc-166886|sc-28709|sc-53496|sc-14691|sc-56594|



RPA32 (phospho Thr21) Polyclonal Antibody

Catalogue No.



Human, Mouse


Phospho-RPA32 (T21) Polyclonal Antibody detects endogenous levels of RPA32 protein only when phosphorylated at T21.


Synthesized phospho-peptide derived from RPA32 (phospho Thr21) at AA range 10-90





Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;







Molecular weight

35 kDa


RPA32 (phospho Thr21) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


RPA32 (phospho Thr21) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Replication protein A 32 kDa subunit antibody, RP-A p32 antibody, Replication factor A protein 2 antibody, RF-A protein 2 antibody, Replication protein A 34 kDa subunit antibody, RP-A p34 antibody

Database links

Human UniProt/Swiss-Prot:P15927;Rat UniProt/Swiss-Port: Q63528;Human Entrez Gene: 6118;Mouse Entrez Gene: 19891;Rat Entrez Gene: Rn.40389

Protein names

Replication protein A 32 kDa subunit , RP-A p32 , Replication factor A protein 2 , RF-A protein 2 , Replication protein A 34 kDa subunit , RP-A p34

Protein function

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Protein tissue specificity

Translationally up-regulated in response to DNA damage (at protein level).

Protein sequence and domain

Belongs to the replication factor A protein 2 family. / Contains 1 OB DNA-binding domain.

Protein post-translational modifications

Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence. / DNA damage-induced ‘Lys-63’-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR.

Protein cellular localization

Nucleus / Nucleus > PML body

Research area

All research areas>Transcription Regulators>Replication Protein A
(View all antibody categories related to Transcription Regulators)


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St John’s Laboratory Ltd.

Product type

Primary antibody


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