Rabbit Polyclonal Phospho-Rpb1 (S1619) antibody (STJ91031)

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Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Rpb1 (S1619) antibody (STJ91031)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rpb1 (phospho Ser1619) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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POLR2A antibody, POLR2 antibody,|DNA directed RNA polymerase II subunit RPB1 antibody|hRPB220 antibody|hsRPB1 antibody|POLR2 antibody|POLR2A antibody|POLRA antibody|polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibody|RNA polymerase II subunit 1 antibody|RNA polymerase II subunit B1 antibody|RP02 antibody|RPB220 antibody|RPBh1 antibody|RpIILS antibody|RPO21 antibody|RPOL2 antibody|SUA8 antibody|Anti-RNA polymerase II RPB1 antibody [EPR1509Y] – ChIP Grade (ab76123)
SCBT cat No: sc-71917|sc-21750|sc-56767|sc-17798|sc-5943|sc-900|sc-47701|sc-55492|sc-9001|sc-65884|sc-271309|sc-28711|

 

Name

Rpb1 (phospho Ser1619) Polyclonal Antibody

Catalogue No.

STJ91031

Reactivity

Human, Mouse, Rat, Monkey

Specificity

Phospho-Rpb1 (S1619) Polyclonal Antibody detects endogenous levels of Rpb1 protein only when phosphorylated at S1619.

Immunogen

Synthesized phospho-peptide derived from Rpb1 (phospho Ser1619) at AA range 1560-1640

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

250 kDa

Formulation

Rpb1 (phospho Ser1619) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Rpb1 (phospho Ser1619) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

DNA-directed RNA polymerase II subunit RPB1 antibody, RNA polymerase II subunit B1 antibody, DNA-directed RNA polymerase II subunit A antibody, DNA-directed RNA polymerase III largest subunit antibody, RNA-directed RNA polymerase II subunit RPB1 antibody,

Database links

Human UniProt/Swiss-Prot:P24928;Mouse UniPort/Swiss-Prot: P08775;Rat UniProt/Swiss-Port: D4A5A6;Human Entrez Gene: 5430;Mouse Entrez Gene: 20020;

Protein names

DNA-directed RNA polymerase II subunit RPB1 , RNA polymerase II subunit B1 , DNA-directed RNA polymerase II subunit A , DNA-directed RNA polymerase III largest subunit , RNA-directed RNA polymerase II subunit RPB1 ,

Protein function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. / Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Protein sequence and domain

The C-terminal domain (CTD) serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. / Belongs to the RNA polymerase beta’ chain family.

Protein post-translational modifications

The tandem heptapeptide repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat and is mediated, at least, by CDK7 and CDK9. CDK7 phosphorylation of POLR2A associated with DNA promotes transcription initiation by triggering dissociation from DNA. Phosphorylation also takes place at ‘Ser-7’ of the heptapeptide repeat, which is required for efficient transcription of snRNA genes and processing of the transcripts. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases, and a ‘CTD code’ that specifies the position of Pol II within the transcription cycle has been proposed. Dephosphorylated by the protein phosphatase CTDSP1. / Among tandem heptapeptide repeats of the C-terminal domain (CTD) some do not match the Y-S-P-T-S-P-S consensus, the seventh serine residue ‘Ser-7’ being replaced by a lysine. ‘Lys-7’ in these non-consensus heptapeptide repeats can be alternatively acetylated, methylated and dimethylated. EP300 is one of the enzyme able to acetylate ‘Lys-7’. Acetylation at ‘Lys-7’ of non-consensus heptapeptide repeats is associated with ‘Ser-2’ phosphorylation and active transcription. It may regulate initiation or early elongation steps of transcription specially for inducible genes. / Methylated at Arg-1810 prior to transcription initiation when the CTD is hypophosphorylated, phosphorylation at Ser-1805 and Ser-1808 preventing this methylation. Symmetrically or asymmetrically dimethylated at Arg-1810 by PRMT5 and CARM1 respectively. Symmetric or asymmetric dimethylation modulates interactions with CTD-binding proteins like SMN1/SMN2 and TDRD3. SMN1/SMN2 interacts preferentially with the symmetrically dimethylated form while TDRD3 interacts with the asymmetric form. Through the recruitment of SMN1/SMN2, symmetric dimethylation is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. CTD dimethylation may also facilitate the expression of select RNAs. Among tandem heptapeptide repeats of the C-terminal domain (CTD) some do not match the Y-S-P-T-S-P-S consensus, the seventh serine residue ‘Ser-7’ being replaced by a lysine. ‘Lys-7’ in these non-consensus heptapeptide repeats can be alternatively acetylated, methylated and dimethylated. Methylation occurs in the earliest transcription stages and precedes or is concomitant to ‘Ser-5’ and ‘Ser-7’ phosphorylation. / Ubiquitinated by WWP2 leading to proteasomal degradation (By similarity). Following UV treatment, the elongating form of RNA polymerase II (RNA pol IIo) is ubiquitinated UV damage sites without leading to degradation: ubiquitination is facilitated by KIAA1530/UVSSA and promotes RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery.

Protein cellular localization

Nucleus

Research area

All research areas>Transcription Regulators>Rpb
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Rpb1 (phospho Ser1619) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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