Rabbit Polyclonal Phospho-RPS6KB1-S424 antibody (AP0253)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-RPS6KB1-S424 antibody (AP0253)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000
IHC 1:50 – 1:100
IF 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

S6K antibody; PS6K antibody; S6K1 antibody; STK14A antibody; p70-S6K antibody; p70 S6KA antibody; p70-alpha antibody; S6K-beta-1 antibody; p70(S6K)-alpha antibody;
70 kDa ribosomal protein S6 kinase 1 antibody|KS6B1_HUMAN antibody|p70 alpha antibody|P70 beta 1 antibody|p70 ribosomal S6 kinase alpha antibody|p70 ribosomal S6 kinase beta 1 antibody|p70 S6 kinase alpha antibody|P70 S6 Kinase antibody|p70 S6 kinase, alpha 1 antibody|p70 S6 kinase, alpha 2 antibody|p70 S6K antibody|p70 S6K-alpha antibody|p70 S6KA antibody|p70(S6K) alpha antibody|p70(S6K)-alpha antibody|p70-alpha antibody|p70-S6K 1 antibody|p70-S6K antibody|P70S6K antibody|P70S6K1 antibody|p70S6Kb antibody|PS6K antibody|Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody|Ribosomal protein S6 kinase beta 1 antibody|Ribosomal protein S6 kinase beta-1 antibody|Ribosomal protein S6 kinase I antibody|RPS6KB1 antibody|S6K antibody|S6K-beta-1 antibody|S6K1 antibody|Serine/threonine kinase 14 alpha antibody|Serine/threonine-protein kinase 14A antibody|STK14A antibody|Anti-S6K1 antibody [E343] (ab32529)

SCBT cat No: sc-393967|sc-230|sc-514501|sc-9027|sc-8418|sc-293269|sc-9381|sc-9379|sc-130833|sc-56190|sc-56191|

Name

Rabbit Polyclonal Phospho-RPS6KB1-S424 antibody

Catalogue No.

AP0253

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding S424 of human RPS6KB1

Host

Rabbit

Applications

WB, IHC, IF

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

56-59kDa

Formulation

Phospho-RPS6KB1-S424 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

S6K antibody; PS6K antibody; S6K1 antibody; STK14A antibody; p70-S6K antibody; p70 S6KA antibody; p70-alpha antibody; S6K-beta-1 antibody; p70(S6K)-alpha antibody;

Protein names

S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha;

Protein function

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.

Protein tissue specificity

Widely expressed.

Protein sequence and domain

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.; Contains 1 AGC-kinase C-terminal domain.; Contains 1 protein kinase domain. The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.; The TOS (TOR signaling) motif is essential for activation by mTORC1.

Protein post-translational modifications

Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.

Protein cellular localization

Cell junction, synapse, synaptosome . Mitochondrion outer membrane. Mitochondrion. Note=Colocalizes with URI1 at mitochondrion.; Isoform Alpha I: Nucleus. Cytoplasm.; Isoform Alpha II: Cytoplasm.

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Kinases-and-Phosphatases/">Kinases and Phosphatases</a>><a href="http://antibodyplus.com/product-tag/p70/">p70</a><br><a href="http://antibodyplus.com/product-tag/Kinases-and-Phosphatases/"&gt; (View all antibody categories related to Kinases and Phosphatases)</a>

Note

AntibodyPlus can customize Phospho-RPS6KB1-S424 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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