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Rabbit Polyclonal Phospho-Separase (S801) antibody (STJ91032)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;
Recommended protocols: check protocols
Click or hover above images to see image description for Separase (phospho Ser801) Polyclonal Antibody.
Check alternative names for the antibodyExpand
ESPL1 antibody, ESP1 antibody, KIAA0165 antibody,|Caspase like protein ESPL1 antibody|Caspase-like protein ESPL1 antibody|ESP 1 antibody|ESP1 antibody|ESPL 1 antibody|Espl1 antibody|ESPL1_HUMAN antibody|Extra spindle poles like 1 antibody|Extra spindle poles like 1 protein antibody|Extra spindle poles-like 1 protein antibody|FLJ46492 antibody|KIAA0165 antibody|Separase antibody|Separin antibody|Similar to fission yeast cut1and gene antibody|SSE antibody|Anti-Separase antibody (ab3762)
SCBT cat No: To be updated
Separase (phospho Ser801) Polyclonal Antibody
|Catalogue No.|| |
Phospho-Separase (S801) Polyclonal Antibody detects endogenous levels of Separase protein only when phosphorylated at S801.
Synthesized phospho-peptide derived from Separase (phospho Ser801) at AA range 740-820
WB, IHC, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;
|Molecular weight|| |
Separase (phospho Ser801) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Separase (phospho Ser801) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Separin antibody, Caspase-like protein ESPL1 antibody, Extra spindle poles-like 1 protein antibody, Separase antibody
|Protein names|| |
Separin , Caspase-like protein ESPL1 , Extra spindle poles-like 1 protein , Separase
|Protein function|| |
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. / All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage. / Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. The association with PTTG1 is not only inhibitory, since PTTG1 is also required for activating it, the enzyme being inactive in cells in which PTTG1 is absent. PTTG1 degradation at anaphase, liberates it and triggers RAD21 cleavage. Second, phosphorylation at Ser-1126 inactivates it. The complete phosphorylation during mitosis, is removed when cells undergo anaphase. Activation of the enzyme at the metaphase-anaphase transition probably requires the removal of both securin and inhibitory phosphate.
|Protein sequence and domain|| |
Contains 1 peptidase C50 domain.
|Protein post-translational modifications|| |
Autocleaves. This function, which is not essential for its protease activity, is unknown. / Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
|Protein cellular localization|| |
Cytoplasm / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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