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Rabbit Polyclonal Phospho-Shc (Y349) antibody (STJ90738)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
SHC1 antibody, SHC antibody, SHCA antibody,|FLJ26504 antibody|p66 antibody|p66SHC antibody|SH2 domain protein C1 antibody|SHC (Src homology 2 domain containing) transforming protein 1 antibody|SHC 1 antibody|SHC A antibody|Shc antibody|SHC transforming protein 1 antibody|SHC transforming protein antibody|SHC-transforming protein 1 antibody|SHC-transforming protein 3 antibody|SHC-transforming protein A antibody|SHC1 antibody|SHC1_HUMAN antibody|SHCA antibody|Src homology 2 domain-containing-transforming protein C1 antibody|Phospho anti-SHC (S36) antibody [6E10] (ab54518)
SCBT cat No: sc-81518|sc-81519|sc-81520|sc-293148|sc-101796|sc-18074|sc-101797|sc-18075|sc-23765|sc-135780|sc-135781|sc-135782|sc-293147|sc-101798|sc-101799|
Shc (phospho Tyr349) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-Shc (Y349) Polyclonal Antibody detects endogenous levels of Shc protein only when phosphorylated at Y349.
Synthesized phospho-peptide derived from Shc (phospho Tyr349) at AA range 290-370
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
|Molecular weight|| |
Shc (phospho Tyr349) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Shc (phospho Tyr349) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
SHC-transforming protein 1 antibody, SHC-transforming protein 3 antibody, SHC-transforming protein A antibody, Src homology 2 domain-containing-transforming protein C1 antibody, SH2 domain protein C1 antibody
|Protein names|| |
SHC-transforming protein 1 , SHC-transforming protein 3 , SHC-transforming protein A , Src homology 2 domain-containing-transforming protein C1 , SH2 domain protein C1
|Protein function|| |
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
|Protein tissue specificity|| |
Widely expressed. Expressed in neural stem cells but absent in mature neurons.
|Protein sequence and domain|| |
In response to a variety of growth factors, isoform p46Shc and isoform p52Shc bind to phosphorylated Trk receptors through their phosphotyrosine binding (PID) and/or SH2 domains. The PID and SH2 domains bind to specific phosphorylated tyrosine residues in the Asn-Pro-Xaa-Tyr(P) motif of the Trk receptors. Isoform p46Shc and isoform p52Shc are in turn phosphorylated on three tyrosine residues within the extended proline-rich domain. These phosphotyrosines act as docking site for GRB2 and thereby are involved in Ras activation (By similarity). / Contains 1 PID domain. / Contains 1 SH2 domain.
|Protein post-translational modifications|| |
Phosphorylated by activated epidermal growth factor receptor. Phosphorylated in response to FLT4 and KIT signaling. Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain. Isoform p66Shc is phosphorylated on Ser-36 by PRKCB upon treatment with insulin, hydrogen peroxide or irradiation with ultraviolet light (By similarity). Tyrosine phosphorylated in response to FLT3 signaling (By similarity). Tyrosine phosphorylated by activated PTK2B/PYK2 (By similarity). Tyrosine phosphorylated by ligand-activated ALK. Tyrosine phosphorylated by ligand-activated PDGFRB. Tyrosine phosphorylated by TEK/TIE2. May be tyrosine phosphorylated by activated PTK2/FAK1; tyrosine phosphorylation was seen in an astrocytoma biopsy, where PTK2/FAK1 kinase activity is high, but not in normal brain tissue. Isoform p52Shc dephosphorylation by PTPN2 may regulate interaction with GRB2.
|Protein cellular localization|| |
Cytoplasm / Mitochondrion matrix / Mitochondrion
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St John’s Laboratory Ltd.
|Product type|| |
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