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Rabbit Polyclonal Phospho-SHC1-Y427 antibody (AP0266)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF
WB 1:500 – 1:2000
IHC 1:50 – 1:100
IF 1:100 – 1:200
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit Polyclonal Phospho-SHC1-Y427 antibody.
Check alternative names for the antibodyExpand
SHC antibody; SHCA antibody;
FLJ26504 antibody|p66 antibody|p66SHC antibody|SH2 domain protein C1 antibody|SHC (Src homology 2 domain containing) transforming protein 1 antibody|SHC 1 antibody|SHC A antibody|Shc antibody|SHC transforming protein 1 antibody|SHC transforming protein antibody|SHC-transforming protein 1 antibody|SHC-transforming protein 3 antibody|SHC-transforming protein A antibody|SHC1 antibody|SHC1_HUMAN antibody|SHCA antibody|Src homology 2 domain-containing-transforming protein C1 antibody|Phospho anti-SHC (S36) antibody [6E10] (ab54518)
SCBT cat No: sc-81518|sc-81519|sc-81520|sc-293148|sc-101796|sc-18074|sc-101797|sc-18075|sc-23765|sc-135780|sc-135781|sc-135782|sc-293147|sc-101798|sc-101799|
Rabbit Polyclonal Phospho-SHC1-Y427 antibody
|Catalogue No.|| |
Human, Mouse, Rat
A phospho specific peptide corresponding to residues surrounding Y427 of human SHC1
WB, IHC, IF
|Recommended dilution|| |
WB 1:500 – 1:2000<br/>IHC 1:50 – 1:100<br/>IF 1:100 – 1:200
Phospho-SHC1-Y427 antibody was tube-contained.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
SHC antibody; SHCA antibody;
|Database links|| |
Human UniProt/Swiss-Prot:<a href="http://www.uniprot.org/uniprot/P29353">P29353</a>
|Protein names|| |
|Protein function|| |
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
|Protein tissue specificity|| |
Widely expressed. Expressed in neural stem cells but absent in mature neurons.
|Protein sequence and domain|| |
Contains 1 PID domain.; Contains 1 SH2 domain. In response to a variety of growth factors, isoform p46Shc and isoform p52Shc bind to phosphorylated Trk receptors through their phosphotyrosine binding (PID) and/or SH2 domains. The PID and SH2 domains bind to specific phosphorylated tyrosine residues in the Asn-Pro-Xaa-Tyr(P) motif of the Trk receptors. Isoform p46Shc and isoform p52Shc are in turn phosphorylated on three tyrosine residues within the extended proline-rich domain. These phosphotyrosines act as docking site for GRB2 and thereby are involved in Ras activation (By similarity).
|Protein post-translational modifications|| |
Phosphorylated by activated epidermal growth factor receptor. Phosphorylated in response to FLT4 and KIT signaling. Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain. Isoform p66Shc is phosphorylated on Ser-36 by PRKCB upon treatment with insulin, hydrogen peroxide or irradiation with ultraviolet light (By similarity). Tyrosine phosphorylated in response to FLT3 signaling (By similarity). Tyrosine phosphorylated by activated PTK2B/PYK2 (By similarity). Tyrosine phosphorylated by ligand-activated ALK. Tyrosine phosphorylated by ligand-activated PDGFRB. Tyrosine phosphorylated by TEK/TIE2. May be tyrosine phosphorylated by activated PTK2/FAK1; tyrosine phosphorylation was seen in an astrocytoma biopsy, where PTK2/FAK1 kinase activity is high, but not in normal brain tissue. Isoform p52Shc dephosphorylation by PTPN2 may regulate interaction with GRB2.
|Protein cellular localization|| |
Cytoplasm.; Isoform p46Shc: Mitochondrion matrix . Note=Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.; Isoform p66Shc: Mitochondrion . Note=In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation.
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Signaling-Intermediates/">Signaling Intermediates</a>><a href="http://antibodyplus.com/product-tag/Shc/">Shc</a><br><a href="http://antibodyplus.com/product-tag/Signaling-Intermediates/"> (View all antibody categories related to Signaling Intermediates)</a>
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|Product type|| |
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