Rabbit polyclonal Phospho-SMC1A-S957 antibody (AP0090)

$69.00$259.00

Reactivity: Human
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-SMC1A-S957 antibody (AP0090)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-SMC1A-S957 antibody.

Alternative names:

Check alternative names for the antibody

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SMC1 antibody, SMCB antibody, CDLS2 antibody, SB1.8 antibody, SMC1L1 antibody, DXS423E antibody, SMC1alpha antibody
CDLS2 antibody|DKFZp686L19178 antibody|DXhXs423e antibody|DXS423E antibody|KIAA0178 antibody|MGC138332 antibody|OTTHUMP00000061876 antibody|RP6 29D12.1 antibody|SB1.8 antibody|Segregation of mitotic chromosomes 1 antibody|Segregation of mitotic chromosomes like 1 antibody|SMC 1 antibody|SMC protein 1B antibody|SMC-1-beta antibody|SMC-1B antibody|SMC1 antibody|SMC1A antibody|SMC1alpha antibody|SMC1alpha protein antibody|SMC1B antibody|SMC1B_HUMAN antibody|SMC1BETA antibody|SMC1beta protein antibody|SMC1L1 antibody|SMC1L2 antibody|SMCB antibody|Structural maintenance of chromosome 1 like 1 protein antibody|Structural maintenance of chromosome 1 like 2 protein antibody|Structural maintenance of chromosomes 1A antibody|Structural maintenance of chromosomes 1B antibody|Structural maintenance of chromosomes protein 1B antibody|Anti-SMC1 antibody (ab21583)

SCBT cat No: sc-71790|sc-56746|sc-71789|sc-243890|sc-135783|sc-135784|sc-166972|sc-98691|sc-100462|sc-166115|sc-166529|

Name

Rabbit polyclonal Phospho-SMC1A-S957 antibody

Catalogue No.

AP0090

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S957 of human SMC1A

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 143kDa/Observed: Refer to Figures

Formulation

Phospho-SMC1A-S957 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-SMC1A-S957 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

SMC1 antibody, SMCB antibody, CDLS2 antibody, SB1.8 antibody, SMC1L1 antibody, DXS423E antibody, SMC1alpha antibody

Database links

Human UniProt/Swiss-Prot:Q14683

Protein names

SMC1, SMCB, CDLS2, SB1.8, SMC1L1, DXS423E, SMC1alpha

Protein function

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Protein sequence and domain

The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure (By similarity).Belongs to the SMC family. SMC1 subfamily.

Protein post-translational modifications

Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.

Protein cellular localization

Nucleus . Chromosome . Chromosome, centromere, kinetochore . Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.

Background

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Research area

All research areas>Transcription Regulators>SMC
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Phospho-SMC1A-S957 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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